基因克隆载体

跨膜蛋白基因克隆载体pMBL的构建及验证

Construction and Verification of the Effectiveness of pMBL : A Cloning Vector of Exported Proteins Encoding Genes

此外，这种新型重组技术可直接将目的基因克隆于载体上，目的基因既可来源于细菌人工染色体也可是基因组DNA。

Furthermore , the new form of recombinogenic engineering is applicable to direct subcloning and cloning of DNA sequences from complex mixtures , including bacterial artificial chromosomes and genomic DNA preparations .

结果酶切和测序证实β2m基因克隆和载体构建成功，目的蛋白在宿主菌中高效表达于包涵体中；

Results Restriction enzyme assay and DNA sequencing showed the β 2 m gene was cloned and the vector was constructed successfully .

重组人活性蛋白C的基因克隆、表达载体构建以及在哺乳动物细胞中的高效表达

Cloning of recombinant human activated protein C ( rhAPC ) gene , constructing expression vector and high expression of rhAPC in mammalian cells

将含脊髓灰质炎病毒（PV）RNA聚合酶的不同长度基因片段克隆到载体pSG5质粒上，分别构建了4个表达RNA聚合酶的质粒。

The four plasmids containing different length of RNA polymerase gene of poliovirus were constructed to express RNA polymerase .

结论成功地构建了HLA-DRα基因片段TA克隆载体。

Conclusion : The HLA-DR α gene TA cloning vector was contracted successfully .

BNYVVCP基因的克隆及载体构建

Cloning of cDNA for BNYVV CP Gene and Construction of Transforming Vector

目的构建含大鼠金属蛋白酶组织抑制物1（tissueinhibitorofmetalloproteinase1，TIMP1）反义基因片段TA克隆载体，为进一步研究TIMP1在肾组织纤维化中的作用奠定基础。

Objective To study the role of TIMP 1 in renal fibrosis , we constructed rat antisense tissue inhibitor of metalloproteinase 1 ( TIMP 1 ) gene TA vector .

并将该基因克隆到pMD18-T载体中，经序列测序，并与GenBank上的14种毒株序列进行比对。

The products of PCR were cloned into pMD-18T vector , sequenced and then the sequences were aligned with 14 sequences from GenBank .

将目的基因克隆到T-载体pMD-18T上，送检测序。

Then the gene was inserted to T - vector ( pMD - 18 T ) .

突变体基因克隆至表达载体pET30a（+）后，利用体外重组实验对表达蛋白的酶活性进行了研究。

Gene of the deletion mutants was then cloned into expression vector pET 30a ( + ) .

方法利用PCR技术扩增tlh基因，克隆至载体pET32a+并测序。将测序结果提交国际互联网上有关生物信息网站进行生物信息学分析。

Methods The tlh gene amplified by PCR was cloned into the vector pET32a + and sequenced , followed by analysis of the biological information by with presenting the sequences to the websites of bioinformatics on the Internet .

目的将恶性疟原虫FCC1/HN株175ku的红细胞结合抗原（EBA-175）基因克隆入测序载体，测定其序列，为以后研究其结构与功能奠定基础。

Objective Sequence the 175 ku erythrocyte binding antigen ( EBA-175 ) of Plasmodium falciparum isolate FCC1 / HN for its structural and functional studies .

大鼠神经营养因子4基因克隆及表达载体的构建

Cloning of neurotrophin 4 gene and the construction of expression vectors in rats

烟草根特异表达启动子的分离鉴定与抗病基因克隆及表达载体构建

Isolation and Characterization of Tobacco Root-specific Promoter and the Constuction of Resistance Gene Plant Expression Vector

FR02全长基因的克隆表达载体的构建及转化苹果

Cloning of the full-length fr 02 gene construction of its expression vector and transformation of it into apple

腐蹄病C型节瘤拟杆菌纤毛蛋白基因的克隆与表达载体的构建

Cloning and Construction Expressing Vector of D.nodosus serotype C Pili Gene

首先，构建克隆载体：将改造的人胰岛素样生长因子（huIGF-I）基因直接克隆到T-载体，并进行DNA序列测定；

First , cloning vector wais constructed : The gene encoding human insulin-like growth factor-I was modified .

结论：①成功地构建了含大鼠TIMP1反义基因片段的TA克隆载体；

Conclusion : ① Rat antisense TIMP-1 gene TA cloning vector was constructed successfully .

PRRS病毒E基因的克隆及表达载体的构建

Cloning of E Gene of PRRS Virus and Construction of Its Expressing Vectors

百合无症病毒CP基因的克隆及其RNAi载体的构建

Cloning of Lily symptomless virus CP Gene and Construction of Its RNAi Vector

玉米蔗糖磷酸合成酶（SPS）基因的克隆及表达载体的构建

Gene cloning of maize sucrose phosphate synthetase and construction of plant expression vector

桃果实中ACC合酶基因克隆及基因沉默载体构建

Cloning of ACC Synthase Gene and Vector Construction of Gene Silencing from Peach

利用PCR法成功地扩增了该基因，并克隆入载体pGEM-T。

The fragment was successfully amplified by PCR and was cloned into pGEM T vector .

扩增2株分离株E基因，克隆到pGEM-T载体进行序列测定，分析变异位点。

The amplified fragments of the envelop gene were cloned into pGEM-T vector and were sequenced .

采用PCR方法，以牛疱疹病毒Ⅰ型基因组DNA为模板扩增gB基因，克隆至pGEM-T载体。

The gB gene was amplified from genomic DNA of bovine herpesvirus-1 by PCR and was cloned into pGEM-T vector .

分别将6个禽痘病毒TK基因克隆至pGEM-Teasy载体中，重组质粒经PCR和酶切鉴定后，进行测序。

The fragment of TK gene of the six APV strains were respectively cloned to pGEM-T Easy vector , and six recombinant plasmids ( pGEM-T-TK ) were obtained .

通过RT-PCR方法扩增出CPA1全酶及活性中心基因，分别克隆入载体pGEM-Teasy，测序分析。

The carboxypeptidase A1 and the active center gene were amplified by RT-PCR from pancreas tissue and cloned into vector pGEM-T easy .

人PD-L1（B7-H1）基因克隆及其逆转录病毒载体的构建和稳定表达

Gene Cloning of Human PD-L1 ( B7-H1 ) and the Corresponding Recombinant Retrovirus Construction and Stable Expression

人源分泌型血管内皮细胞生长因子受体flt-1（Ⅰ～Ⅳ/区）的基因克隆及其腺病毒载体的构建和表达

Gene Cloning of Secreted VEGF Receptor flt-1 (ⅰ~ⅳ extracellular domain ) and The Corresponding Recombinant Adenovirus Construction and Expression