异硫氰酸荧光素

穿膜肽标记异硫氰酸荧光素及钆喷替酸葡甲胺行MR分子显像的探讨

Molecular imaging of a cell-penetrating peptide labeled Fluorescein-5-isothiocyanate and MR contrast agents : Gadopentetate dimeglumine

异硫氰酸荧光素标记红细胞（FITCRBC）技术对胰腺活体进行局部微血管观察。

FITC-RBC was used to observe blood flow in pancreatic microvessels in rats with AEP .

使用HPLC法测定人参皂苷Rb1、阿霉素、异硫氰酸荧光素的载药量，采用分光光度计法测定银耳多糖的载药量。

Determinated drug loading of ginsenosides Rb1 , adriamycin , fluorescein isocyanate by HPLC and Tremella polysaccharide by Spectrophotometer .

并可以装载人参皂苷Rb1、阿霉素、异硫氰酸荧光素、银耳多糖。

And this method can load ginsenoside Rb1 , adriamycin , fluorescein isothiocyanate and Tremella polysaccharide .

方法用异硫氰酸荧光素（FITC）标记法。

Method Used FITC labeled method .

采用异硫氰酸荧光素（FITC）标记的核糖核酸酶（FITCRNase）作为探针，结合半薄切片技术，观察了慢性萎缩性胃炎与正常胃活检组织中RNA的分布变化。

Using FITC labeled RNase as a probe the distribution of RNA in gastric mucosa of patients with Chronic atrophic gastritis was obtained by gastroscopy .

用异硫氰酸荧光素牛血清白蛋白（Fluorescein－isothiocvanatebovineserumalbumin，FITC－BSA）示踪法测定兔眼葡萄膜巩膜途径房水流出量。

Uveoscleral outflow ( Fu ) was measured using fluorescein-isothiocyanate bovine serum albumin ( FITC-BSA ) as the tracer in six normal white rabbits ( 12 eyes ) .

同时采用异硫氰酸荧光素（FITC）标记maltodextrin，以荧光分光光度计检测荧光强度。

At the same time , maltodextrin labeled by fluorescein isothiocyanate ( FITC ) . The fluorescence intensity was detected by fluorescence spectrophotometer . 2 .

载人参皂苷Rb1和银耳多糖的人血白蛋白微粒载药量较高，载阿霉素和异硫氰酸荧光素的人血白蛋白微粒载药量较低。

Ginsenoside Rb1 and Tremella polysaccharides human serum albumin particles have higher drug loading , and adriamycin and fluorescein isothiocyanate human serum albumin particles have lower drug loading .

方法：用异硫氰酸荧光素（FITC）标记LDL，荧光分光光度法测定FITC-LDL的含量；

Methods : LDL was conjugated to fluorescein isothiocyanate ( FITC ) and the fluoro - spectrophotometry was established to quantify the concentration of FITC-LDL ;

巢蛋白抗体IgG，β-微管蛋白Ⅲ抗体IgG，胶质纤维酸性蛋白抗体IgG，生物素标记二抗和三抗、异硫氰酸荧光素标记二抗。

Nestin antibody IgG , β - microtubule protein ⅲ antibody IgG , glial fibrillary acidic protein antibody IgG , biotin labeled second antibody and third antibody and fluorescinisothiocyate ( FITC ) - labeled second antibody were used in this experiment .

研究了不同载体、不同标记物（包括辣根过氧化物酶（HRP），Cy3，异硫氰酸荧光素（FITC），胶体金和生物素）标记单抗用作检测抗体、芯片保存时间等对检测灵敏度的影响。

We investigated influence of the supports , markers ( Cy3 , Horseradish peroxidase , FITC , colloidal gold and biotin ) and storage on antibody microarray sensitivity .

异硫氰酸荧光素标记的葡聚糖（FITC-DX）摄入试验检测AML-DC的液相大分子吞噬功能。

Fluorescein isothiocyanate-conjugated dextran ( FITC-DX ) uptake test examined the fluid phase macromolecule uptake function of cultured AML cells .

其异硫氰酸荧光素（FITC）荧光标记抗体筛选灵敏度可达体积稀释比1∶2000。采用氩离子激光扫描荧光成像技术，成功实现了基于光谱自编码微球的多样品免疫荧光混合筛选和荧光成像。

The volume dilution rate of FITC assay in screening can reach 1 ∶ 2000.Applying Ar ~ + - laser fluorescence scan-imaging technology , immunofluorescence mix-screening of multi-samples and fluorescence imaging based on optical self-encoding microbeads was realized successfully .

提出了一种基于异硫氰酸荧光素（FITC）标记荧光抗体修饰介孔SiO2的超灵敏标记探针，并成功用于检测人血清中甲胎蛋白（AFP）的含量。

1.4 . A novel ultra-sensitive immunoassay probe was proposed based on fluorescein isothiocyanate ( FITC ) labeled antibody modified mesoporous materials . The prepared signal amplification label has been successfully used to detect the level of α - fetoprotein ( AFP ) in human serum samples .

标记了异硫氰酸荧光素（FITC）的IgE适配体与IgE结合后，阻碍了抗异硫氰酸荧光素抗体（anti-FITC）与FITC的结合而产生FITC的荧光，通过检测荧光强度实现对IgE的定量检测。

The binding of IgE with its aptamer labeled with fluorescein isothiocyanate ( FITC ) hampered the binding of anti-fluorescein isothiocyanate ( anti-FITC ), which reserves the fluorescence emission of FITC . Through detecting the fluorescent intensity of FITC , we could detect IgE quantitatively .

用异硫氰酸荧光素（FITC）标记EB病毒（EBV）感染体外培养的人胚鼻咽上皮和鼻咽癌细胞株（CNE-1和CNE-2），对照为EBV受体阳性的Raji和Ramos细胞。

The in vitro cultured human embryonic nasopharyngeal epithelium and nasopharyngeal carcinoma epithelial cell lines ( CNE1 and CNE2 ) were infected with Epstein-Barr virus ( EBV ) labeled with fluorescein isothiocyanate ( FITC ) . Raji and Ramos cells were used as controls because they were EBV-receptor positive .

本文探讨应用两株高特异性配对C-PMCAb分别标记125I和异硫氰酸荧光素（FITC），采用抗FITC磁性微粒子固相抗体作为分离剂，建立血清C-PIRMA检测法。

The serum C-P liquid phase immunoradiometric assay ( IRMA ) with tow labeled antibodies was developed , using two high specific C-P McAb strains labeled with () ~ ( 125 ) I and FITC respectively and Anti-FITC Ab coupled to magnetic particles added during reaction process as separate reagent .

以2BN90和辣根过氧化物酶、异硫氰酸荧光素的结合物进行直接酶联免疫吸附试验和直接荧光抗体试验，均获得成功。

Both the direct enzyme-linked immunosorbent assay ( ELISA ) and the direct immunofluorescence test ( IF ) for MDV were established with horseradish peroxidase ( HRP ) and fluorescein isothiocyanate ( FITC ) labeled McAb " 2BN90 " .

用异硫氰酸荧光素-葡聚糖（FITC-Dextran）示踪法检测大鼠肠粘膜通透性，免疫荧光法检测肠粘膜组织紧密连接蛋白ZO-1、F-肌动蛋白及p-MLC表达。

The intestinal permeability of rats was measured by fluorescein-isothiocyanate-dextran ( FITC-Dextran ) method . The changes of tight junction protein ZO-1 , cytoskeleton F-actin , and the protein expression of phosphorylated MLC ( p-MLC ) in intestinal epithelia of the rats were analyzed by immunofluorescence . 2 .

异硫氰酸荧光素-牛血清白蛋白标记物的化学发光反应

Study on Chemiluminescence Reaction of Fluorescein Isothiocyanate Labelled Protein by Flow Injection Method

抗马干扰素-γ单克隆抗体的异硫氰酸荧光素标记及应用

Preparation and Application of Equine IFN - γ Monoclonal Antibody Labeled with FITC

对异硫氰酸荧光素等水溶性较差药物，本方法也有一定的局限性。

For some drugs which like FITC with less water-soluble , this method also has some limitations .

异硫氰酸荧光素标记抗体的固体基质室温磷光性质研究

Study on the Properties of Solid Substrate Room Temperature Phosphorescence of the Antibody Labeled with Fluorescein Isothiocynate

阳性对照组：异硫氰酸荧光素标记的α-银环蛇毒素（FITC-α-Bgt）标记巨噬细胞；

Positive control : macrophages treated with FITC labeled α - Bgt ( FITC - α - Bgt ) .

将新型材料用异硫氰酸荧光素标记，激光共聚焦显微镜观察其细胞内定位。

The GC-PEI polymer was labeled by FITC . The localization of the polymer in cell was observed using confocal microscopy .

用破伤风毒素和异硫氰酸荧光素标记马抗破伤风血清可以特异地显示体外培养的神经细胞。

Tetanus toxin and FITC labelled horse anti-tetanus toxoid serum were applied to show the plasma membrane of cultivated chicken embryonic cerebral cortical cells .