整合表达

透明颤菌vgb基因在枯草芽孢杆菌中的整合表达

Integrated expression and roles of vitreoscilla hemoglobin gene ( vgb ) in Bacillus subtilis

枯草芽孢杆菌麦芽糖启动子P△glvA整合表达载体的构建及启动子功能的初步探讨

Construction of the Integrative Expression Vector for the Glv Operon Promoter P △ glvA of Bacillus Subtilis and a Preliminary Discussion on Promoter Function

受体后代与供体在同工酶图谱上的相似性以及供体外观性状在受体后代中的高比例表达证实外源DNA己经导入受体，并得到整合表达。

The similarity of two isoenzyme zymograms between descendents of receptor and donor and the expression of external characters of donor in descendents can be indicators of the success of introduction of exogenous DNA into receptor and the success of expression of exogenous DNA in the descendents .

猪导入生长激素基因的整合表达和遗传

Integration , Expression and Transmisson of Growth Hormone Genes Introduced into Pigs

番茄质体多顺反子定点整合表达载体的构建及其转烟草的研究

Construction of Tomato Chloroplast Multicistron Site Integration Expression Vector and Its Transgenic Tobacco

小麦叶绿体基因组定点整合表达载体的构建

The Construction of Site-specific Integration and Expression Vector for Chloroplast Genome of Wheat

安全环保的烟草质体多顺反子定点整合表达载体的构建

Construction of The Safely and Friendly Environmental Tobacco Chloroplast Multicistron Site Integration Expression Vector

脱胶关键酶基因的克隆及其在黑曲霉中的整合表达

Gene Cloning of the Crucial Degumming Enzyme and Its Integration and Expression in Aspergillus niger

菊苣质体同源片段的克隆及非抗生素标记的质体定点整合表达载体构建

Cloning of homologous targeting sequences and construction of antibiotic-free plastid site-specific integration expression vector of chicory

突变野油菜黄单胞菌α-淀粉酶基因在酿酒酵母中的整合表达

Integration expression of a mutated alpha-amylase gene from Xanthomonas campestris in the ribosomal DNA of Saccharomyces cerevisiae

但是，外源基因的有效导入、整合表达水平及遗传稳定性上还存在很多不足。

However it still has many disadvantages , including effect induction , expression of exogenous gene and steady heredity of transgenic individual .

目的尝试水稻质体多顺反子定点整合表达载体转化到烟草质体中表达。

【 Objective 】 The rice ( Oryza sativa ) chloroplast multicistron site integration expression vector will be transformed for expression into the tobacco chloroplast .

转基因三倍体毛白杨外源基因及其表达的检测转基因白桦中外源基因整合表达稳定性与遗传变异分析

The Detection on the Foreign Gene and Expression of the Transgenic Triploid of Chinese White Poplar Stability of Integration and Expression of Foreign Gene in Transgenic Birch and Analysis of Genetic Variation

Southern和Northern杂交进一步证实了目的基因的正确整合和表达。

Southern and Northern blot further indicate that the integration and expression of inserted genes were correct .

用PCR、半定量RT-PCR检测各组基因的整合及表达。

Extraneous gene integration and expression were confirmed by PCR and semi-quantitative RT-PCR .

PCR、Elisa法鉴定并检测TNFα基因的整合和表达。

The integration and expression of TNF - α gene were analyzed by PCR and ELISA .

应用PCR和RT-PCR方法检测TK基因的整合及表达；

The methods of PCR and RT-PCR were used to confirm existence and expression of TK gene .

红系特异的GFP基因在转基因小鼠中的整合和表达

Integration and expression of GFP gene directed by the erythroid-specific element in transgenic mice

结果RT-PCR及Southernblot分析证明tk基因在人喉癌细胞系Hep-2中有整合及表达；

Results RT-PCR and Southern blot analysis confirmed the integration and expression of tk gene in Hep-2 cells .

pGH基因在F1代转基因金鱼中的整合及表达的分析

Analysis of Integration and Expression of pGH Gene in F_ ( 1 ) - Generation Transgenic Goldfish

经Southernblot和Northern－blot分析证实在小鼠BMMC中具有β－珠蛋白基因的整合与表达。

Southern blot analysis and Northern-blot analysis showed that human β - globin gene was integrated and expressed in BMMC .

人IL-2Rα基因在CHO细胞中的稳定整合和表达及其应用

Studies on the Stable Integration and Expression of IL-2R α Gene in CHO Cells and It ′ s Applications

方法原核显微注射法制备HBV转基因小鼠，用巢式PCR、Southern杂交、免疫组织化学、ELISA等检测HBV基因的整合与表达。

Methods HBV transgenic mice models were produced by microinjection to analyze the integration , expression of HBV in the transgenic mice by nested PCR , southern blot , immunohistochemistry , and ELISA .

对转化细胞系采用Southernblot、NorthernBlot及westernblot杂交分析，证实有稳定的外源基因的整合及表达，而内源性TGFα的表达及合成均

The integration of retrovirus vector and expression of exogenous gene in the transformant PC-7 cells were confirmed by Southern blot , Northern blot and Western blot hybridizations . The gene expression and protein

∕（ICR）BR小鼠受精卵中，并移植到假孕受体鼠的输卵管内，然后应用PCR、westernblot杂交技术分析子代鼠中基因的整合及表达情况。

∕( ICR ) BR mice and transplanted into the oviducts of pseudopregnancy female mice . The integration and expression of exogenous genes in offspring were detected by PCR and Western Blot , respectively .

方法将携带HBx基因的表达质粒pHAHBx转染HepG2肝癌细胞，G418筛选阳性细胞克隆，RTPCR鉴定HBx基因的整合及表达；

Methods The plasmid pHA-HBx carrying HBx gene was transfected into HepG_2 cells , and the positive clones were screened and identified with G418 and RT-PCR , respectively .

人小肠三叶因子（hITF）基因在生菜中的整合与表达

Expression of Human Intestinal Trefoil Factor ( hITF ) Gene in Lettuce

说明外源基因在胚胎早期发育阶段可以表达，EGFP可以作为报告基因来实现对外源基因整合及表达的监测。

The results implied that EGFP gene could be used as reporter gene to check expression of foreign gene in the transgenic goat cloned embryos in early stages .

根癌农杆菌将T-DNA转移并插入寄主细胞的基因组，需要趋化与附着、vir区活化、T-DNA切割及转运、T-DNA整合及表达等步骤。

Agrobacterium tumefaciens-mediated transformation included steps such as chemotaxis , attachment , vir region induction , T-DNA processing , transport , insertion , genes expression and so on .

人类白细胞抗原B2704基因在转基因小鼠中的整合与表达

Integration and expression of HLA-B 2704 gene in transgenic mice