显微注射

将3株诱捕ES细胞分别经显微注射引入到受体囊胚中，再植入假孕母鼠的子宫中使其发育成小鼠。

ES cells from 3 trapped lines were introduced into blastocysts by microinjection .

采用显微注射法，将GFP基因注入FVB/NJ小鼠受精卵原核内，获得子代鼠。

GFP gene was introduced into the zygote of FVB / NJ mice by microinjection .

微量牛卵中H1fooCDS序列的克隆及其mRNA向卵内显微注射

Cloning of H1 foo CDS from Trace Bovine Oocytes and Micro-injection of H1 foo mRNA into Oocyte

显微注射法建立HCV5'NCR和C区转基因细胞模型以及核酶细胞内抑制HCV基因表达作用的研究

Establishment of Transgenic Cell Lines for HCV 5'NCR , C by Microinjection and Inhibition of HCV Gene Expression by Hammerhead Ribozymes

通过显微注射MO，分别特异性敲降这两个重复基因，将导致不同的发育畸形。

Distinct developmental defects are observed when specifically knockdown each of these two duplicated genes by corresponding MO injection .

回顾了目前利用微机电（MEMS）技术在高效并行显微注射和胚胎定位方面的工作；

In this paper , we reviewed our work on high - throughput microinjection and positioning of embryos using the MEMS technologies .

另外，内源ABA（通过显微注射）也可诱导保卫细胞产生H2O2，进而促进气孔关闭，这些效应同样可通过显微注射CAT或DPI被部分逆转。

In addition , ABA microinjected into guard cells markedly induced H2O2 production , which preceded stomatal closure .

对采用外源基因原核显微注射法生产的基因转移山羊，用一种新的DNA非同位素标记法即地高辛配基标记DNA探针作外源基因整合检测。

Gene transfer goats produced by the method of microinjection foreign gene into pronuclear were made integrated detection with a novel nonradioactive DNA labeling method , namely DNA probe labeling with Digoxigenin .

用常规显微注射法将鲤鱼总DNA注入鲮鱼受精卵，并将受精卵培育成鱼种。鳙小卫星DNA的克隆

In this paper , We microinjected carp DNA into mud carp ( Cirrhina molitorella ) fertilized eggs , and then established the experimental mud carp . Cloning of minisatellite DNA from bighead carp

圆形精细胞卵胞质内显微注射（roundspermatidinjection，ROSI）辅助生殖技术的出现为治疗人类男性不育提供了新的途径。

The appearance of Round spermatid injection ( ROSI ) provides a new way to treat the sterile human male .

结果在显微注射所产生的转基因小鼠中，有4只为Southernblot检测阳性，并且在这4只小鼠及其子代中均检测到人bcl-xL的mRNA和过表达的目的蛋白。

Results In the transgenic mice after microinjection , there were 4 Southern-blot positive mice , and the expression of bcl-xL mRNA and protein was detected .

用显微注射方法，将线性化的载体DNA注入KM×B6D2F1小鼠受精卵的的原核内部，然后将胚胎移植到输卵管内，13只受体怀孕产仔。

Then the linear vector was microinjected into the pronucleus of the KM × B6D2F1 zygotes to produce the transgenic mice .

方法原核显微注射法制备HBV转基因小鼠，用巢式PCR、Southern杂交、免疫组织化学、ELISA等检测HBV基因的整合与表达。

Methods HBV transgenic mice models were produced by microinjection to analyze the integration , expression of HBV in the transgenic mice by nested PCR , southern blot , immunohistochemistry , and ELISA .

显微注射2～5个ES细胞的成活率和孵化率均较高，分别为90.90%和78.79%。

When microinjected with 2 ~ 5 ES cells , the rates of embryo survival and hatch were both the highest , being 90.90 % and 78.79 % .

采用显微注射法，将所提取的RNA注射于成熟的爪蟾卵母细胞中，采用电压钳位方法记录表达到非洲爪蟾卵母细胞膜上的神经递质受体通道电流。

The mature oocytes of clawed frog were injected with the extracted RNA by microinjection , and double electrode voltage clamps were used to detect activated peak currents of expressed neurotransmitter receptor channel .

并应用cAMP及蛋白激酶抑制剂（PKI）显微注射入1细胞期受精卵内，观察卵细胞形态学变化及卵裂率、死亡率。

Morphological changes and percentage of cleavage or death were observed after microinjection of PKA stimulator cAMP and protein kinase inhibitor ( PKI ) into 1-cell stage fertilized eggs of mice .

目的比较3种早期停用曲普瑞林方案与改良的曲普瑞林长方案在体外受精-胚胎移植（IVF-ET）和卵母细胞单精子显微注射（ICSI）中的效果。

Objective To compare the efficacy of 3 early cessation protocols with the modified long protocol of triptorelin in IVF-ET and ICSI .

目的研究人bcl-xL基因在显微注射所产生的子代小鼠中的整合、转录和表达情况。

Objective To study the integration , transcription and expression of human bcl-xL gene in the transgenic mice generated by microinjection .

目的：探讨采用卵胞浆内单精子显微注射（ICSI）治疗少、弱、畸精子不育症的临床效果。

Objective : To study the clinical effect of intracytoplasmic sperm injection ( ICSI ) in treatment of oligozoospermia , azoospermia and teratozoospermia .

既往卵母细胞单精子显微注射（ICSI）不良结局和有限的第2次有丝分裂中期卵细胞的患者应用激光辅助的ICSI

Use of laser-assisted intracytoplasmic sperm injection ( ICSI ) in patients with a history of poor ICSI outcome and limited metaphase II oocytes

用显微注射法建立转bcl-xl基因小鼠

Developing bcl-x_l transgenic mice by microinjection

结果表明：1显微注射外源基因后以单细胞前、中期孵化率最高.与对照组比差异不显著（P＞0.05）.与其它组比较差异极显著（P＜0.01）；

The results showed that : 1.The incubation rates were the highest at prophase and metaphase of one-cell , and there was no significant difference from the control ( P > 0.05 ) . but there was very significant difference from other batches ( P < 0.01 );

显微注射法建立含荧光素酶和HCV-C基因细胞模型

Establishment of cell model expressing HCV-C and luciferase fusion protein by microinjection

小鼠原核卵显微注射MT-hGH融合基因后的早期发育

The Early Development of MT-hGH Fusion Gene Microinjected Mouse Pronuclear Eggs

方法：显微注射AlphaMyHCPDGFD质粒后，产生的PDGFD转基因c57小鼠养至4～6周。

Methods : Transgenic mice were generated by microinjection of purified transgenic Alpha MyHC PDGF D vector into the pronuclei of fertilized eggs .

结果显微注射法和乳腺注射法转基因后，t-PA可在小鼠和牛的乳汁中表达。

Results t-PA was detected in the milks of mice and cows after the transgenic manipulation with microinjection and mammary gland injection of the fusion gene .

为了优化转基因小鼠的制备技术，试验着重对注射DNA的制备、高质量受精卵的获得、显微注射、胚胎移植及早期胚胎检测等各步骤中的主要影响因素进行了优化。

To optimize the technique of transgenic mice making , in this research , the major steps such as the preparation of DNA fragments for microinjection , the preparation of eggs with high quality , the microinjection and implantation and early embryos detection were optimized .

显微注射11～15个ES细胞和未经显微注射的胚胎成活率分别为95.12%和82.14%，差异极显著（P<0.01）；

When microinjected with 11 ~ 15 ES cells and no microinjection , the rates of embryo survival were 95.12 % and 82.14 % , the difference was extremely significant ( P < 0.01 );

4将卵子去除颗粒细胞，安装显微注射针，调试显微操作仪，挑选成熟度卵母细胞进行单精子胞浆注射（ICSI）。

Remove granulosa cells of egg , installing micro-injection needle , commissioning a micromanipulator , selecting the maturity of oocytes to sperm cytoplasmic injection ( ICSI ) .

为建立对HBsAg耐受的小鼠模型，利用显微注射转基因技术，构建了CMV启动子控制的HBsAg（ayw亚型）转基因小鼠。

To construct a murine model that is immunotolerant to HBsAg , we developed the HBsAg ( ayw subtype ) transgenic ( Tg ) mice with CMV promoter by microinjection .