显微注射法

用显微注射法将绿色荧光蛋白基因导入金鱼受精卵中表达

Expression of GFP gene transferred into the fertilized eggs of Carassius auratus by microinjection

采用显微注射法，将GFP基因注入FVB/NJ小鼠受精卵原核内，获得子代鼠。

GFP gene was introduced into the zygote of FVB / NJ mice by microinjection .

显微注射法建立HCV5'NCR和C区转基因细胞模型以及核酶细胞内抑制HCV基因表达作用的研究

Establishment of Transgenic Cell Lines for HCV 5'NCR , C by Microinjection and Inhibition of HCV Gene Expression by Hammerhead Ribozymes

对采用外源基因原核显微注射法生产的基因转移山羊，用一种新的DNA非同位素标记法即地高辛配基标记DNA探针作外源基因整合检测。

Gene transfer goats produced by the method of microinjection foreign gene into pronuclear were made integrated detection with a novel nonradioactive DNA labeling method , namely DNA probe labeling with Digoxigenin .

用常规显微注射法将鲤鱼总DNA注入鲮鱼受精卵，并将受精卵培育成鱼种。鳙小卫星DNA的克隆

In this paper , We microinjected carp DNA into mud carp ( Cirrhina molitorella ) fertilized eggs , and then established the experimental mud carp . Cloning of minisatellite DNA from bighead carp

采用显微注射法，将所提取的RNA注射于成熟的爪蟾卵母细胞中，采用电压钳位方法记录表达到非洲爪蟾卵母细胞膜上的神经递质受体通道电流。

The mature oocytes of clawed frog were injected with the extracted RNA by microinjection , and double electrode voltage clamps were used to detect activated peak currents of expressed neurotransmitter receptor channel .

用显微注射法建立转bcl-xl基因小鼠

Developing bcl-x_l transgenic mice by microinjection

显微注射法建立含荧光素酶和HCV-C基因细胞模型

Establishment of cell model expressing HCV-C and luciferase fusion protein by microinjection

结果显微注射法和乳腺注射法转基因后，t-PA可在小鼠和牛的乳汁中表达。

Results t-PA was detected in the milks of mice and cows after the transgenic manipulation with microinjection and mammary gland injection of the fusion gene .

结论：通过显微注射法使外源基因pEF1-αmINS在小鼠基因组中得到整合，建立了人胰岛素的转基因小鼠模型。

Conclusion : pEF1 α - mINS fragment can integrate into mice genomic DNA . An animal model of transgenic mice expressing human insulin can be successfully established .

方法采用显微注射法，将人bcl-xL基因注入昆明小白鼠受精卵获得子代鼠，然后作PCR、Southern-blot、mRNA、Western-blot检测以获得阳性鼠。

Methods Bcl xL gene was introduced into mice by microinjection . The presence of bcl xL and its expression were confirmed by PCR , Southern blot , mRNA inspection , Western blot in the founders .

用显微注射法将Epstein－Bar病毒（EBV）的潜伏膜蛋白（LMP）基因分组注入1239只昆明种小白鼠受精卵的原核中。

N order to study the function of nasopharyngeal carcinoma related gene-latent membraneprotein ( LMP ) gene of EB virus in human body , the male pronuclei of 1239 zygotes from Kun-ming white mice have been microinjected with LMP gene .

为进一步深入研究NR5A2的生理功能，本研究通过原核显微注射法构建nr5a2转基因小鼠。

To facilitate the further functional studies of NR5A2 , we plan to establish a nr5 α 2 transgenic mouse by using pronucleus microinjection .

方法：原核显微注射法产生转基因小鼠。

METHODS : Transgenic mice were produced by pronu-clei microinjection method .

非桔抗基因（转移基因的表达产物互不抑制）的共转移（显微注射法）是一种既经济、又有效的方法。

Coinjection is a simple gene transfer method which saves time and money .

采用显微注射法和乳腺注射法将融合基因转入小鼠的受精卵和小鼠及牛的乳腺组织中。

The fusion gene was then transferred into the mouse zygote and the mammary gland tissue of mice and cows .

背景和目的：目前实验室制备转基因动物常用方法仍然是原核显微注射法。

Background and purpose : Preparation of the current laboratory methods of transgenic animals is still commonly use pronuclear injection .

受精卵原核显微注射法是当前最常用最有效的建立转基因动物的方法之一。

A method for microinjection of mouse zygotes to produce transgenic mice is the commonly used and effectively transfered method in present .

方法：用显微注射法把小鼠附睾头和附睾尾精子注入卵母细胞的胞质内或卵周隙进行显微受精。

Methods : ICSI and SUZI were employed to obtain the assisted fertilization of mouse sperm from caput epididymidis and cauda epididymidis .

在建立遗传工程小鼠模型的众多方法中，显微注射法是目前国际上公认的制备转基因及基因剔除动物模型的首选。

Among the several techniques for establishing genetically modified mice , micro injection approach is considered as the first choice to generate genetically modified mouse models .

转基因是近年来在生命科学领域广泛应用的新兴技术，目前80%以上的转基因动物模型是利用显微注射法构建完成的。

Gene transfer is a new and widely used technology in bioscience field . At present over 80 percent of transgenic animal models are constructed by means of microinjection .

结论经巩膜外路至视网膜下腔的显微注射法是较为理想的视网膜下腔注射给药和视网膜细胞移植方式。用于外层视网膜病变的表层型人工视网膜技术（英文）

Conclusions This specific technique of subretinal microinjection is an easy , safe and optimal technique to meet the need of subretinal drug administration and retinal cells transplantation for retinal degenerative diseases .

传统的制作转基因动物方法有显微注射法、逆转录病毒感染法和胚胎干细胞法等，但每种方法都有其缺陷，限制了其在今后转基因动物研究中的广泛应用。

Microinjection , retrovirus mediated method and embryonic stem cells method were all the traditional methods of producing transgenic animals . But these methods had defects and limited application in the research of transgenic animals in the future .

然后通过显微注射法将Hu-IFN-α重组基因注射入草鱼1-2细胞期的受精卵内，从而开始了转基因草鱼抗病育种的研究。

Then , by the use of the micro - injection technology , we injected the reorganized HU-IFN-a gene into the grass carps ' fertilized eggs at its 1-2 cell period to cultivate the trans-HU-IFN - α gene grass carp .