机械分离

方法采用机械分离、酶消化和不连续密度梯度离心法分离提取TIL前体细胞；

Methods TIL were obtained through mechanical separation , enzyme digestion and discontinuous density gradient centrifugation .

采用酶解和机械分离相结合的方法，急性分离新生大鼠海马CA3区锥体神经元。

Combining the methods of enzymic digestion and mechanical separation , rat hippocampal CA3 pyramidal neurons can be separated from the slice of the brain tissue .

用膜片箝技术的细胞贴附式（cellattached）和内面向外式（insideout），在机械分离的新生大鼠的大脑皮层神经元上，记录到ATP激活的单通道电流。

The ATP-activated single channel currents were recorded on single neuron dissociated from the cerebral cortex of neonatal tat with the meChanical method by the Cell-attached and inSide-out configurations of patch clamp technique .

方法采用酶消化法及酶辅助机械分离法分别分离IPE和RPE细胞。

Methods IPE and RPE cells were isolated by enzymatic digestion method and enzyme-assisted microdissection method respectively .

通过采用机械分离和消化分离相结合的方法分离不同胎龄大鼠脑NSC。

Methods NSCs of different fetal age rats were isolated and cultured through the combination of mechanical and pancreatin digestant isolation .

目的采用机械分离和酶消化相结合的方法分离小肠上皮类器官单位（IOUs），并进行形态及功能鉴定，建立稳定的分离培养技术。

Objective To establish a method on isolating intestinal epithelium organoid units ( IOUs ) and identify the morphological integrity and viability .

使用酶消化和机械分离出成年美洲蜚蠊的腹部末端神经节（TAG），然后在无菌条件下分离DUM神经元细胞。

Isolation of adult DUM neurons was performed under sterile conditions using enzymatic digestion and mechanical dissociation of the median parts of the TAG from the nerve cord of the cockroach Periplaneta Americana .

用机械分离法从豚鼠前庭感觉上皮中分离出不同种类的前庭感觉毛细胞（VHC）。

Living vestibular hair cells ( VHCs ) were separated from vestibular sensory epithelium of guinea pig by micromanipulation .

方法取新生1周内乳鼠的大脑皮质，机械分离出神经干细胞，无血清培养液（DMEM/F12+bFGF+EGF+B27）培养，免疫组化SABC法对神经干细胞进行鉴定；

Methods NSCs harvested from the cerebral cortex of neonatal one-week mice were triturated and cultivated in serum-free DMEM / F12 + bFGF + EGF + B27 , then identified by the immunohistochemistry SABC method .

方法利用木瓜蛋白酶消化及机械分离法分离出豚鼠鼻粘膜单个纤毛细胞，用配备相差或微分干涉相衬（diferentialinterferencecontrast，DIC）装置的倒置显微镜在100倍油镜下（总放大率1000倍）观察。

Methods The ciliated cells were isolated by means of mechanical dissociation after papain treatment and a differential interference contrast ( DIC ) microscope was used for 1000-fold observation ( objective lens : 100-fold ; eye piece : 10-fold ) .

目的建立机械分离并培养小鼠耳蜗螺旋神经节神经元（SGN）细胞模型的方法。

Objective To conduct a study on the methods for establishing a cytological model of mechanical dissociation and culture of spiral ganglion neurons ( SGNs ) in postnatal mice .

方法：分离出生1d大鼠海马，吸管吹打机械分离制成单细胞悬液，采用无血清培养和胎牛血清诱导分化，应用免疫荧光技术鉴定神经干细胞及其分化的子代细胞。

Methods : Hippocampi of neonatal rats ( 1 day ) were taken out and mechanically dissociated into single cell suspension . After serum-free cultivation and fetal bovine serum-induced proliferation , neural stem cells and their progeny cells were identified by immunofluorescence technique .

结论成功建立了机械分离并培养SGN细胞模型的方法，SGN存活细胞的数量和周期可以满足体外多种实验的需要。

Conclusion We successfully established the methods of mechanical dissociation and culture of SGN cytological model ; the cell amount and survival period of SGNs were able to meet the requirement of cytological experiments .

从7只豚鼠前庭中取出壶腹嵴、位觉斑，放置在胶原酶溶液中孵育50min～60min后行机械分离。

The crista ampullaris and maculae acusticae were isolated from the vestibular organs of 7 guinea pigs . They were treated with collagenase for 50 or 60 minutes , then separated mechanically .

方法取出生后第1天的大鼠耳蜗，利用机械分离和酶消化相结合的方法分离出纯的GER细胞，于含10%血清DMEM培基中进行培养。

METHODS An experimental method was developed which allowed the isolation and culture of GER cells from P1 rat cochleae using a combinatorial approach of enzymatic digestion and mechanical separation . The dissociated GER cells were cultured in DMEM + 10 % FBS .

方法取胎龄8~12周的人胚胎脑海马，机械分离成单细胞悬液，以2×106个细胞/ml接种到含h-EGF、h-bFGF和h-LIF的DMEM/F12培养液中。

Methods The cells from hippocampus of aborted human embryo tissue ( 8-12 weeks after conception ) were mechanically dissociated and cultured with DMEM / F 12 medium containing h-EGF , h-bFGF , h-LIF .

方法显微机械分离孕10～11d胚胎大鼠脊髓神经干细胞，无血清悬浮培养，以复合添加剂N4定向诱导分化，并拍照记录不同时期细胞分化的形态；

Methods SNSCs were isolated mechanically from rat embryonic ( E10 to 11d ) spinal cord under microscope . SNSCs were cultured and maintained in serum-free medium . Directional differentiation from SNSCs to neurocytes was induced by adding N4 supplement .

方法从胚胎大鼠脑皮质中机械分离细胞，应用N2培养基进行培养和扩增，采用免疫组织化学方法进行鉴定，应用Ad5βgal重组腺病毒载体检测神经干细胞表达外源基因的能力。

Methods The cells from the embryonic rat cortices were mechanically dissociated and the N2 medium was adopted to culture and expand the cells . Then the cells were identified by immunocytochemistry method , and the Ad5 β gal vector was applied to test their capacity of expressing foreign gene .

结果新生小鼠SGN在机械分离、体外培养条件下，可以获得良好的细胞形态和轴突再生能力，其存活细胞的数量为（78.10±4.33）/cm2（5～7d），生长周期为7～14d。

Results In the state of primary culture in vitro , the SGNs were found to have good cell morphology and axon regeneration power , the surviving cells of SGN ( 5-7 d ) amounted to ( 78.10 ± 4.33 ) / cm 2 and the survival period was 7-14 d.

目的通过机械分离胰腺的方法，提高成人胰岛数量和质量。

Objective To improve adult islet isolation quality with mechanization method .

机械分离的果蝇幼虫中枢神经元全细胞钾电流的特性

Properties of whole-cell potassium currents in mechanically dissociated Drosophila larval central neurons

结论：酶辅助机械分离法是进行虹膜色素上皮细胞原代培养较好的方法。

Conclusion : It is a good method to detach IPE by enzyme-assisted microdissection .

牛腔前卵泡的简易机械分离方法

A simple Mechanical method for the isolation of intact preantral follicles from adult bovine

机械分离并培养螺旋神经节神经元方法的建立

Establishment of Methods of Mechanical Dissociation and Culture of Spiral Ganglion Neurons in Postnatal Mice

酶-机械分离-酶消化法分离培养兔眼虹膜色素上皮细胞

Isolation and culture of rabbit 's iris pigment epithelial cells by enzyme-microdissection-enzyme digestion method in vitro

方法：采用低浓度、短时间胰酶消化和机械分离相结合的方法制备皮层神经元悬液，进行培养。

Low concentration and shortened time of trypsin digestion and mechanical dissociation were adopted to conduct culture .

新疆卡拉库尔羊腔前卵泡体外机械分离与采集的一种新方法

A New Mechanical Method for the Isolation and Collection of Preantral Follicles from Ovaries of XinJiang Karakul Sheep

方法：采用酶辅助机械分离法分离并培养兔眼虹膜色素上皮细胞。

Methods : The IPE of rabbits were detached and cultivated according to the method of enzyme-assisted microdissection .

方法采用机械分离法、胰蛋白酶消化分离法和胶原酶消化分离法，分别对手术切除的喉鳞状细胞癌标本进行单细胞分离。

Methods We dissociated the carcinoma cells from resected laryngeal squamous carcinoma specimen by physical , trypsin and collagenase methods .

目的对耳蜗灌注法和酶解加机械分离法的结果进行比较，并观察单离毛细胞的形态和存活情况。

Objective To compare the methods for isolating hair cells and study the viability and morphology of the isolated cells .