核心序列
- 名core sequence
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这两个序列中含有多个复制转录因子的结合位点、增强子基序、多个完全的和非完全的反向重复序列以及潜在的DNA弯曲核心序列样结构。
Both the MAR loci show numerous replication / transcription factor binding sites , enhancer motifs , several perfect or imperfect inverted repeats , and sequences sharing the common features of the potential DNA bending core sequence .
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综合分析得56个微卫星核心序列,52条微卫星序列已提交NCBI数据库。
Comprehensive analysis of 56 core sequence of microsatellites , 52 microsatellite sequences have been submitted to NCBI database .
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人SRY基因核心序列的扩增和分析
Amplification and Analysis of the Highly Conserved Region of Human SRY Gene
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方法采用酵母单杂交体系筛选与GPE核心序列相互作用的转录激活因子,应用DNA序列测定及计算机分析等手段对所测的DNA序列进行分析。
Methods We searched the trans action factors binding to GPE ⅰ using yeast one hybrid system , and analyzed the DNA sequence by sequencing and computer search .
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SSR标记,即简单序列重复,根据核心序列串联重复数目不同,利用两侧的DNA序列作引物,对包含重复序列的基因组进行PCR扩增,检测DNA的变异。
SSR ( simple sequence repeat ) can detect the DNA mutation by using the DNA sequence of both sides of a section containing different tandem repeat core sequence as primers and amplifying the genome DNA containing repeat sequence .
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方法:应用酵母单杂交体系,以3重复串连的SPⅠ核心序列为诱饵,对酵母细胞中的人肝细胞cDNA文库进行筛选,应用DNA序列测定及生物信息学方法对筛选出的结果进行分析。
Methods : Yeast one-hybrid system was employed in screening of a human liver cDNA library using 3 copies of SP I core sequence as a bait . And the results were analyzed by sequencing and BLAST .
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LCR由4个DNase历高敏位点(5'HS1-5'HS4)构成,每个HS的活性又都集中在核心序列上。
The β - globin gene LCR consists of four erythroid-specific DNase I hypersensitive sites ( HS1-HS4 ), every HS activity is defined to its core sequences .
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病毒载体介导的携带位点控制区中单个或多个DNaseⅠ高敏位点核心序列的人β-珠蛋白基因在鼠红系细胞中的整合与表达
Integration and Expression of the Human β - Globin Gene with Single or Multiple DNase ⅰ Hypersensitive Site Core Fragments from Locus Control Region Following Viral Vectors Mediated Gene Transfer into Mouse Erythroid Cells
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结论大鼠c-Jun蛋白和线粒体腺苷酸转位酶在酵母细胞内与大鼠GST-P增强子GPE核心序列结合,可能是作用于GPE的反式作用因子。
Conclusions Rat c Jun and mitochondrial adenine nucleotide translocase can bind to the GPE ⅰ core sequence , and they perhaps are the trans action factors of GPE ⅰ .
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通过温度梯度试验,确定出各微卫星引物的最适合退火温度分别为:33.15微卫星核心序列,54℃;(CAC)5,57℃。
Through the gradient temperature test , we got the fittest annealing temperature of each microsatellite primer : the 33.15 core sequence primer 's was 54 ℃ and the ( CAC ) _5 primers ' was 57 ℃ .
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由于不同STRs位点的核心序列不同,而同一STR位点在不同人中的重复次数不同,导致出现个体差异,构成了长度多态性,广泛应用于个体识别和亲权鉴定。
Different STRs have different core sequences , and the same STRs have different repetitions among persons . These lead to the individual differences and form a genetic polymorphism . So STRs are widely used in individual identification and paternity testing .
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构成作品的基本方式是核心序列的分割,从而揭示了这首作品从内核到外延,从局部到整体,从和声到整体结构的逻辑关系。
It showed the work logic relationship from kernel to extension , from part to whole , and from harmony to the integral structure .
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根据多种鳞翅目昆虫NPVsod基因的保守区序列设计引物,克隆了柞蚕NPV的sod基因中300个核苷酸的核心区序列。
A pair of primers based on conservative sequences of some lepidopteran nuclear polyhedrosis virus superoxide dismutase genes was designed to amplify the core fragment of Antheraea pernyi NPV sod gene .
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2007年,三雄极光将金卤灯,T5支架,灯杯,镇流器,天花灯,格栅射灯,支架领域开发新产品,扩大核心产品序列。
In2007 , three will be hung Aurora Metal Halide , T5 , lamp cup , ballast , Tin Lantern , grille spotlights and stent area to develop new products , the core product expansion Serialization .
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人β1整合素启动子核心启动序列初步分析
The Preliminary Analysis of Core Sequences at Human Integrin β 1 Promoter
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通过测序确认基因座核心重复序列及等位基因核心序列重复数。
We have confirmed the core repeats and its repeating number of these alleles by sequencing .
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丙型肝炎病毒基因分型及核心区序列同源性分析
Genotyping of hepatitis C virus and homology analysis of its core gene sequences isolated from different geological regions
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通过比较各个模型预测性能,可知建立模型时需要考虑核心结合序列两侧的扩展位置上氨基酸的影响。
Through compared among the predictive performance of models , it is known that the adjacent positions of core binding sequence need to be considered .
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结果所得HCV核心区cDNA序列为503bp。
Results The HCV cDNA sequence of core region was ( 503 bp ) .
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第二,以GUI动作为核心生成事件序列,并程序化地生成测试用例。
Secondly , GUI action is the key to generate sequence of events , and generate test case by procedure .
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总共37个来自不同物种的DNA聚合酶X家族成员的核心结构域序列被分析。
A total of 37 DNA polymerase core domain sequences ( SMART domain ), which come from numerous species with different evolutional levels , including above two virus members , were analyzed .
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经分析发现这些内含子富含A·T,具有类似启动子和HSE核心结构的序列存在。
Analysis of these introns indicated that they are rich in A · T , have similar structure of promoter and core structure of the HSE .
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而核心启动子序列中的转录因子SP1结合位点具有与肝癌相关细胞系的细胞核蛋白结合的活性。
The oligonucleotides that contain the SP1 binding sites in the core promoter can bind to the nuclear extracts of hepatocellular related cells .
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对于这两种预测外源基因的方法,CGS是利用核心基因的序列特征信息预测外源基因,而MGC是利用基因在染色体上的位置去预测外源基因。
Of the two proposed methods , CGS uses the information of sequence characteristics to predict alien genes and MGC is utilizing gene position on a chromosome to predict alien genes .
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研究表明,这种序列可激活多种免疫效应细胞,其特征结构如CpG核心、侧翼序列、骨架长度等都对其免疫刺激特性有重要影响。
Some studies Showed that these motifs are able to stimulate immune cells , and that the specific structures ( such as CpG core , terminal sequences , the length of skeleton framework and so on ) have significant effect to immunostimulatory function .
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蛋白质核心设计的序列组合文库筛选方法
Design of Protein Cores by Screening Combinatorial Sequence Library
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乙型肝炎病毒核心抗原基因序列的启动子活性
The promoter activity of DNA sequences within HBcAg gene of hepatitis B virus ADR nc-1
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针对基因组序列搜索的特点,从提高序列搜索效率出发,提出了一种新的、速度更快的搜索方法,其核心是通过序列特征的分析和比较搜索相似序列。
To make the genome sequence searching more efficient , we proposed a new search method with high speed . The idea is to search similar sequence by analyzing and comparing the sequence feature .
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在以前的版本中,RM协议的核心思想在于消息序列的概念。
As in previous revisions , the essence of the RM protocol is the concept of a Sequence of messages .
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另一个为随机引物,针对外显子和内含子的特点,设计为分别富含GC或AT核心区的任意序列。
The second primer , arbitrary primer , is an arbitrary sequence with either an AT - or GC-rich core to anneal with an intron or extron , respectively .