测序仪

应用DNA测序仪定量检测mRNA的方法研究

Study on the Method of Quantitation of mRNA by DNA Sequencer

所有多重PCR进一步合并为17个可在ABI337测序仪上进行电泳的组合。

All multiplexes were further combined into 17 sets for the electrophoresis on ABI 377 sequencer .

那时他们还没有发明出测序仪，是吧？

They hadn 't invented sequencers back in those days , had they ?

在收购CompleteGenomics后，这可能会发生变化，因为华大基因计划引入新型基因测序仪。

That may change with the purchase of Complete Genomics as BGI plans to introduce a new sequencing machine .

在用测序仪对DNA测序前要对其进行复制和荧光标记。

For DNA , copies of the DNA to be sequenced are made and labeled with fluorescent markers before they are identified using a sequencing machine .

应用377自动测序仪及相关软件对ER-α基因TA重复微卫星多态性进行基因分型。

Genotyping of the ER-alpha microsatellite polymorphism was performed using ABI 377 automated sequencer and affiliated software .

通过ABI310或3100测序仪检测，在所观察到的20个基因型比例（genotypicratios）（10个微卫星位点×2个家系）中，有17个基因型比例符合孟德尔遗传。

Of the 20 genotypic ratios observed ( 10 loci x 2 crosses ), 17 ratios conformed to Mendelian segregation .

在实验室中，承担这类工作的设备称为DNA测序仪，有了它，科学家们就可以精确地定位突变，并针对患者个体的遗传特征量身打造个性化治疗，极大地促进了生物医学研究和发现。

The devices used by the laboratories , known as DNA sequencers , have greatly accelerated biomedical research and discovery , allowing scientists to pinpoint mutations and devise treatments tailored to the genetic characteristics of individual patients .

方法采用1对Cyt-b基因通用引物对人和19种动物共171例样本的mtDNA进行PCR扩增，琼脂糖凝胶检测扩增产物，ABI377测序仪及荧光测序技术分析扩增产物的DNA序列。

Methods mtDNA of 171 samples from human to 19 species of animals were tested by a pair of universal cyto b PCR primer .

用Edman法在蛋白测序仪上测出SFL的N端肽链30个氨基酸的排列顺序为：T/A/VDXLXFTFSDFDPNGEDLLFQGDAHVTSNN。

It was determined by Edman that the sequence of the N_terminal thirty amino acids was : T / A / VDXLXFTFSDFDPNGEDLLFQGDAHVTSNN .

15个微卫星DNA用荧光标记引物复合扩增技术和DNA自动测序仪进行分型，比较受者移植前后或供者的DNA图谱，观察受者移植后DNA图谱的变化。

DNA samples were amplified using a fluorescent labeling primers multiplex amplification system of 15 microsatellites markers , followed by typing on a DNA automated sequencer . Recipients ' or donors ' microsatellites DNA profiles were compared before and after transplantation .

随即对扩增的DNA行单链构像多态性（SSCP）分析。而后将突变或异常的SSCP产物分离后，采用自动荧光测序仪检测DNA序列。

Single-strand conformational polymorphism ( SSCP ) analysis of the amplified DNA was performed to screen for mutations and aberrant SSCP products were isolated and sequenced by dye terminator cycle sequencing method using an automated fluorescent sequencer .

Chelex法提取DNA，PCR扩增，非变性聚丙烯酰胺凝胶不连续缓冲系统水平电泳分型，自动激光荧光测序仪测定DNA序列。

The DNA samples were extracted with Chelex method and were amplified by PCR technique . The PCR products were analyzed by the PAG horizontal electrophoresis with discontinuous buffer system and by the approach of the automated fluorescence detection .

如果你想要用一种迥然不同的方式去考察基因组，哈佛大学干细胞与再生生物学系（DepartmentofStemCellandRegenerativeBiology）乱糟糟地放满了试验台、测序仪和显微镜的迷宫般的三楼是一个绝佳场所。

If you want to see the genome in a fundamentally different way , the best place to go is the third floor of Harvard 's Department of Stem Cell and Regenerative Biology , in a maze of cluttered benches , sequencing machines and microscopes .

方法：经静脉采血2mL，常规提取基因组DNA，以PCR扩增GCH-1基因，反应产物用自动DNA测序仪直接测序。

METHODS : Venous blood ( 2 mL ) was drawn and genomic DNA was extracted by standard technique . GCH 1 genes were amplified by PCR , and reaction products were directly sequenced by DNA sequencer .

华大基因是Illumina最大单一客户，占其DNA测序仪订单的40%，每台设备售价高达50万美元。

BGI was the single biggest customer for Illumina , accounting for as much as 40 per cent of its DNA sequencing machine orders , for equipment that cost up to $ 500,000 apiece .

方法用聚合酶链式反应（PCR）方法从人类胎肝cDNA基因文库中分离出全长cDNA克隆，用DNA自动测序仪双方向测序，体外酵母细胞中表达蛋白质。

Methods A cDNA was isolated by touchdown PCR from a cDNA library of human fetal liver using specific primers . The DNA sequencing was done by an auto-sequence machine through forward and reverse directions . The expression of cDNA encoded protein was tested in yeast cells .

方法采用PCR技术从金黄色葡萄球菌染色体DNA上扩增得到SEB基因，克隆到原核表达载体pET22b（+）上，转化大肠杆菌BL21（DE3）感受态细胞，全自动测序仪测序。

Methods The full-length of SEB gene amplified from DNA of S6 strain of Staphylococcus aureus by high-fidelity PCR was cloned into prokaryotic expression vector pET22b ~ + , and then the vector was transformed into BL21 ( DE3 ) to construct a prokaryotic expression system .

负责公司分子与细胞部门测序仪在指定区域的销售。

Responsible for the sale of Solid products in certain Area .

方法：自动蛋白测序仪法。

Methods : By using automatic analyzer for protein sequencing .

这个纳米孔测序仪只有传统基因序列测定仪的万分之一大小。

This nanopore-based sequencer is one 10000th the size of your traditional genome sequencer .

用PE-ABD491A蛋白质N-末端测序仪测定其N-末端氨基酸排列顺序是N-Met-Arg-Thr-Gln-Glu-His-Thr-Lys-Ser-Val。

Its N-terminal sequence was determined to be N-Met-Arg-Thr-Gln-Glu-His-Thr-Lys - Ser-Val by PE-ABD 491A Protein N-Terminal Sequencing Meter .

我们现在有能力使用基因测序仪来反复读取这种病毒的遗传密码。

We now have the ability to use genetic sequencing machines to read the code of the virus again and again .

在该公司里面，150多台先进基因测序仪正每天对数千个人类基因组进行分析。

Inside , more than 150 state of the art genetic sequencing machines were analysing the equivalent of thousands of human genomes a day .

本研究首先深度分析中国高通量测序仪的市场特点，市场发展趋势预测，市场的机会和未来可能面临的挑战，同时分析了目前中国客户分类和其消费心理和消费习惯。

This paper deeply analyzed the high throughput sequencing market , including market trend , market opportunities and challenges as well . This paper also analyzed the customer profile , consuming psychology , and spending habit .

我们现在拥有的许多新工具，是上世纪九十年代完全没有的。我们现在有能力使用基因测序仪来反复读取这种病毒的遗传密码。

We have a whole bunch of new tools that we never had in the 1990s . We now have the ability to use genetic sequencing machines to read the code of the virus again and again .

重组体用克隆位点上游和下游的T7和T3启动子序列为测序引物，用自动测序仪测序鉴定克隆的正确性。

The recombinant was sequenced by automatic sequencer with promoters T7 in upstream and T3 in downstream as sequencing primers .

测序电泳和数据收集在DNA测序仪上进行。

Sequencing electrophoresis and data collection were performed on DNA sequencer .

背景：由于测序和片段分析中的先进技术，DNA测序仪和分析仪在短时间内就产生了大量的数据。

BACKGROUND : Due to the advanced techniques in sequencing and fragment analysis , DNA sequencers and analyzers produce vast amounts of data within short time .