测序分析

茎特异表达基因EST测序分析及RNA微阵列验证

EST Sequencing and RNA Array Analysis of Stem Specific Genes

新疆蒙古族肝炎患者的TTV检测、克隆与测序分析

Cloning and sequencing of TTV in Mongolian patients with hepatitis in Xinjiang

线粒体DNA测序分析及其法医学应用

Mitochondrial DNA Sequencing Analysis and Its Application in Forensic Science

结果：构建的融合基因经过DNA测序分析；

RESULTS : The mito-MPG fusion gene was confirmed by DNA sequencing ;

优化单链DNA模板的制备条件和焦磷酸测序分析的参数。

Single-stranded DNA template preparation and pyrosequencing analysis of the parameters were optimized .

常规方法对质粒进行鉴定和DNA测序分析。

The harvested recombinant plasmid was identified by conventional methods and DNA sequencing .

结果：PAGE电泳和DNA测序分析证实定向插入的寡聚核苷酸串与预先设计的插入序列一致。

Results : Both PAGE electropheresis and DNA sequence analysis confirmed the inserted oligonucleotides .

成年发病狼疮鼠的T细胞受体Vβ基因测序分析

Sequence analysis of T cell receptor V β genes in aged lupus mice

结果酶切证实目的DNA定向克隆至载体上，测序分析结果与目的序列相同。

Results The target DNA was directly cloned to vector and the result was correct by sequence analysis .

采用RACE技术克隆了CsHRP的全长cDNA并测序分析。

RACE was used to clone the full-lenth cDNA of the gene .

选择其中最大的一个克隆进行了测序分析，发现这是一个新的cDNA序列。

The sequence of cDNA clone with the largest insert was analyzed .

并对重组质粒进行双酶切、菌落PCR及测序分析进行鉴定。

To verified the by restriction enzyme digestion , bacterial colony PCR , and sequencing analysis .

方法用p表型细胞和抗PP1PK（-Tja）血清进行鉴定，并进行测序分析。

Method P blood group system was identified using p phenotype cells , anti PP 1 P k antiserum , and direct DNA sequencing .

不同肺腺癌细胞系N－ras、p53基因突变核酸测序分析

Sequence analysis of N-ras and p53 gene mutation in the human lung adenocarcinoma cell lines

对阳性PCR结果进行测序分析，并与GenBank上的序列进行比较。

Positive PCR products were sequenced and compared with those from the GenBank .

五氯酚诱导斑马鱼P53基因外显子7点突变的克隆测序分析

Cloning and Sequencing Analysis of Point Mutation of p53 Gene Exon 7 Induced by Pentachlorophenol in zebrafish

通过cDNA测序分析剪切位点突变引起编码序列的变化。

The cDNA sequencing was performed to analyze the change of FV coding sequence by splice site mutation .

重度感染家蚕微孢子虫的家蚕丝腺cDNA文库构建及EST测序分析

CDNA Library Construction and EST Analysis of Bombyx mori Silk Gland Heavily Infected by Nosema bombycis

经测序分析结果显示，铁蛋白重链在正向消减cDNA文库中有2个阳性克隆。

Sequencing analysis showed that there were two clones contained ferritin H chain in the forward subtractive cDNA library .

结果：构建了M-A杂合肽基因重组质粒，经PCR扩增、酶切和DNA测序分析表明，杂合肽基因的DNA序列及阅读框完全正确。

Results : PCR amplification and DNA sequencing analysis confirmed that hybrid peptide gene was correctly inserted into the vector .

PCR和DNA测序分析质粒ampC基因型；

The genotypes of AmpC β - lactamases were determined by polymerase chain reaction and DNA sequencing . Plasmid was extracted by alkaline lyses .

人肝癌细胞系HLE细胞表面抗原多肽的denovo测序分析

De novo Sequencing of Antigen-derived Peptides Expressed on the Surface of HLE Cells

HLA基因测序分析进一步肯定了HLAI类PCRSSP分型的特异性。

The HLA gene sequencing results of some special cases further confirmed the specificity of HLA class I PCR SSP typing results .

PCR扩增研究对象PDS基因第6、9外显子，对PCR扩增产物直接测序分析。

Exons 6 and 9 of the PDS gene in all subjects were amplified by polymerase chain reaction and analyzed by direct DNA sequencing .

山羊痘病毒基因组提取、酶切及TK基因克隆测序分析

Extraction and Restriction Endonuclease Analysis of Genomic DNA and Sequence Analysis of TK Gene from Goatpox Virus

随机挑取37个克隆进行测序分析，发现了12个新的EST。

37 clones randomly picked out were sequenced , in which 12 were novel expressed sequence tags ( ESTs ) .

经PCR产物测序分析，该引物扩增片段的第387位点上存在C→T突变，但氨基酸没有发生改变都为甘氨酸。

PCR product by sequencing , the amplified fragment of the first 387 points , the existence of C → T mutation , but there is no change of amino acid for glycine .

再次设计两对引物，通过重叠延伸PCR对该序列进行定点诱变，经测序分析，获得了序列完全正确的PG全长基因。

The full-length gene was site-directed mutagenesis by overlap extension PCR , and the PCR products were sequenced to obtain the correct sequence . 2 .

口腔鳞癌K-ras基因测序分析及p21~（ras）蛋白的表达

Sequence analysis of K-ras and p21 ~ ( ras ) protein ex - pression in oral squamous cell carcinomas

用PCR技术扩增GBC-SD细胞p53基因4～9外显子，对所得产物进行直接测序分析。

Exons 4 ~ 9 of the p53 gene were amplified using PCR technique , and the amplification products were direct sequencing analysed .