激光共聚焦扫描显微镜

应用免疫组化和免疫荧光双标技术结合激光共聚焦扫描显微镜，观察缺血性脑损伤后脑内nestin的表达及其细胞类型。

Immunohistochemistry and double immunofluorescent labeling techniques combined with confocal laser scanning microscope analysis were used to investigate the characteristic spatial induction profile of nestin following a transient middle cerebral artery occlusion in adult rat brain .

利用免疫细胞化学方法在激光共聚焦扫描显微镜下观察该细胞表面抗原的表达情况，进行鉴定。

To observe the cell surface antigen of rat EHSCs with the confocal laser scanning microscope , using immunocytochemistry for identification .

利用激光共聚焦扫描显微镜结合各种荧光染料探针和荧光共振能量转移（FluorescenceResonanceEnergyTransfer，FRET）技术研究了激光照射生物效应的分子机制。

The mechanism of the effect was investigated using specific fluorescent probes staining and fluorescence resonance energy transfer ( FRET ) techniques .

采用免疫组织化学和激光共聚焦扫描显微镜技术，观察大鼠比目鱼肌神经肌肉接头部NCAM的分布变化。

The distribution of N-CAM in neuromuscular junctions of soleus muscle in rats was examined with immunohistochemical method and confocal laser scanning microscopy technique .

方法：应用MTT法、流式细胞术和激光共聚焦扫描显微镜技术，体外观察该化合物对JAR细胞的影响。

Methods : MTT assay , flow cytometry and laser scanning confocal microscopy were used to detect the effects of4-MS on JAR cells .

方法：采用免疫荧光技术对22例正常食管粘膜、20例癌旁组织和23例癌组织中LC进行标记，通过激光共聚焦扫描显微镜对LC进行断层扫描和三维立体结构重建。

Method : LCs in normal mucosa , mucosa adjacent to cancer and cancer tissues were labeled with immunofluorescence method and were scanned and reconstructed with laser scanning confocal microscope .

在MRc-600型激光共聚焦扫描显微镜下看到了清晰的绿色荧光，表明gfp基因获得了克隆与表达。

Less bright green fluorescence observed under a con-focal laser scanning microscope showed that green fluorescent protein ( gfp ) gene was cloned and expressed well .

采用激光共聚焦扫描显微镜观察Phospho-JNK蛋白在细胞内的定位与分布情况。

The localization and distribution of phosphorylation-JNK were observed by confocal laser scanning microscopy . 4 .

激光共聚焦扫描显微镜分析显示nestin阳性细胞同样表达NSE和CFAP，证实了缺血性脑损伤可诱导神经干细胞分化，有利于神经元和星形胶质细胞再生。

Double staining also showed that nestin-positive cells co-expressed with GFAP and / or NSE . The results verified that ischemic damage in adult brain induced the expression of nestin and neural stem cells may differentiate into neuron and / or astrocyte after ischemia .

用BFA处理百合花粉管后，通过免疫荧光标记及激光共聚焦扫描显微镜观察发现类细胞质力蛋白中间链对BFA不敏感；同样的处理发现类红膜肽对BFA敏感。

After treatment with BFA , immunoflurescence labeling and confocal laser scanning microscopy indicated that the cytoplasmic dynein intermediate chain-like protein in lily pollen tube was insensitive to BFA treating , but spectrin-like protein was sensitive to it under the same condition .

方法：（1）在聚苯乙烯塑料片上分别形成3,12,20小时变链菌生物膜标本，用荧光染料FITC-ConA对胞外多糖进行染色，激光共聚焦扫描显微镜观察。

Methods : ( 1 ) S.mutans biofilms which were formed on polystyrene plastic sheets in 3 , 12 and 20 hours respectively , exopolysaccharides distribution were visualized by fluorescent FITC-ConA stained with confocal laser scanning microscopy .

不同发育阶段大鼠不同大动脉光镜及激光共聚焦扫描显微镜观察

Study of Rat 's Large Artery with LM and CLSM on Different Development

壳聚糖-海藻酸聚电解质复合膜（Ⅰ）激光共聚焦扫描显微镜分析形貌

Chitosan-alginate polyelectrolyte complex membrane ( I ) Topography analysis by laser confocal scanning microscopy

方法应用免疫荧光组织化学双标记染色技术，在激光共聚焦扫描显微镜下观察染色结果。

Methods Double label immunofluorescence histochemical staining and confocal laser_scanning microscope were used in the experiment .

应用免疫荧光染色法，激光共聚焦扫描显微镜观察细胞间粘附分子1（ICAM1）的表达。

The expression of ICAM 1 was determined by immunofluorescent staining method with confocal laser scanning fluorescence microscope .

倒置显微镜、光学显微镜和激光共聚焦扫描显微镜观察PGLH7细胞凋亡的形态学特征并进行凋亡细胞鉴定；

The apoptosis morphological changes were observed under the inverted microscope , light microscope and fluorescence microscope in vitro .

方法采用激光共聚焦扫描显微镜和流式细胞技术进行观察与检测。

Methods Confocal laser scanning microscope ( CLSM ) and flow cytometry ( FCM ) were used in this study .

激光共聚焦扫描显微镜定量分析细胞内游离钙离子浓度。

Cell cycle was detected with flow cytometry , and the concentration of free intracellular calcium was examined with laser scanning confocal microscopy .

激光共聚焦扫描显微镜动态观察体外培养大鼠皮层神经元受氯化亚铁作用时过氧化物水平及膜电位的变化

Changes of peroxide level and membrane potential in laser confocal scanning microscope after in vitro cultured cortex neurons were exposed to ferrous chloride

完成激光共聚焦扫描显微镜产品鉴定样机的大部分软件，申请软件发明专利和实用新型专利。

Developed most parts of the whole software for the laser confocal scanning microscopy system . Applied for the software invention patent and the practical patent .

用标记菌株接种限菌培养条件下生长的玉米（农大3318）幼苗，在接种8天、12天后，取其根部，用激光共聚焦扫描显微镜进行观测。

Observations were performed with confocal laser scanning microscopy ( CLSM ) and electron microscopy , respectively , at 8 d and 12 d / 10d after inoculation .

应用激光共聚焦扫描显微镜及荧光蛋白基因融合技术观测了细胞生长过程中调节基因的时空表达规律。

Confocal laser scanning microscopy was applied to observe the temporal and spatial expressions during cell cultivation for the mutants with the fusion between regulative genes and a fluorescent protein gene .

目前主要使用激光共聚焦扫描显微镜观察绿色荧光蛋白的表达，但需要昂贵的仪器并耗费大量时间。

In this study , we developed a new system of laser-induced fluorescence ( LIF ) for microfluidic chip applied to detecting the expression of green fluorescent protein ( GFP ) in Bacillus subtilis .

为了给我国在生物医学、基因工程等领域进行不懈研究的广大科学工作者提供强有力的研究工具，更好地赶超世界前沿水平，从而着手研制开发激光共聚焦扫描显微镜系统和基因芯片扫描仪。

In order to provide strong researching tools for the researchers in those fields to reach and exceed the world advanced level , we have developed Laser confocal scanning microscopy and Gene chip scanner .

方法：以Fluo-4/AM荧光指示剂负载培养的心肌细胞，应用激光共聚焦扫描显微镜检测胞浆和核Ca2+震荡的变化。

METHODS : Rat neonatal cardiac myocytes were cultured , and fluo-4 / AM was loaded as calcium probe . The changes of cytosolic and nuclear Ca 2 + were observed by confocal laser microscopy .

水稻子房经固定、脱水、包埋、切片、真空粘片法粘片、荧光抗体标记等处理后，在激光共聚焦扫描显微镜下观察胚囊中的微管并拍照。

Ovaries of rice were conducted by fixing , dehydrating , embedding by PEG in small centrifuge tube , cutting section by razor , vacuum gluing section , labeling by fluorescence antibody , and then microtubules in embryo sac were observed and photographed by confocal laser scanning microscopy .

利用原子力显微镜（AFM）、透射电子显微镜（TEM）以及激光共聚焦扫描荧光显微镜观察了磁场对离体受体聚集和对CHL细胞上受体聚集的影响。

By using atomic force microscope ( AFM ), transmission electron microscope ( TEM ), and con-focal laser scanning fluorescent microscope , the effects of ELF-MF on the clustering of purified receptors , and of the receptors in CHL cell membrane have investigated .

对光学显微镜以及电子显微镜的发展进行了回顾，重点介绍了自20世纪以来显微镜技术的重大进展，并对激光共聚焦显微镜和扫描隧道显微镜进行了重点介绍。

The development of optical microscope and electron microscope is reviewed in this paper , with emphasis on introduction of important development of microscope technology since the 20th century , especially on Laser Scanning Confocal Microscope and Scanning Tunneling Microscope .