编码区连接
编码区连接-
将4个含有不同大小启动子区的克隆片段与GUS基因编码区连接构建成嵌合基因，通过基因枪轰击转入香蕉叶、根和果实的细胞后。
The 5 ′ - flanking fragments in different length were fused to the coding sequence of GUS gene . These constructs were delivered to leaf , root and fruit cells of banana via particle bombardment .
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将这些缺失片段分别与gus基因编码区连接，构建成融合质粒，经土壤农杆菌（Agrobacterium）介导引入水稻，定量测定转基因水稻植株未成熟种子中的gus酶活力。
Each of the chimeric genes consisting of these deleted sequences and coding sequence of β - glucuronidase ( gus ) gene was respectively introduced into rice plants mediated by Agrobacterium and the expression of the chimeric gene in immature seeds of transgenic plants was examined ( Fig.3 ) .