虫荧光素酶

固定化虫荧光素酶反应器测定ATP的初步研究

Determination of concentration of ATP by immobilized firefly luciferase reactor

根据萤火虫荧光素酶的结构特点和性质，对ATP生物发光反应中酶的稳定性进行研究，研制出新型酶保护剂，提高了ATP生物发光检测技术的稳定性和灵敏度。

According to the structure and characteristics of firefly luciferase , the stability of enzyme was studied . New enzyme protectants were developed , which made the stability and sensitivity of the ATP bioluminescent detection techniques were greatly improved .

以虫荧光素酶法检验了四株丝状真菌在葡萄糖&无机盐液体培养过程中的胞内ATP含量。

The intracellular ATP contents in 4 strains of mycelial fungi , grown on the glucose-inorganic salts medium , were determined by luciferin-luciferase system .

WB和虫荧光素酶报告实验表明，Nef在体外可以特异性的下调前列腺癌细胞中的AR的表达水平以及PSA启动子的活性。

The consequences of WB and Luciferase Reporter Assay showed that Nef down-regulated AR expression level and PSA promoter activity .

运用KSHVRta基因启动子（KSHV复制时最先被激活的启动子）驱动的虫荧光素酶报告基因进一步证实并扩展研究结果。

The results were extended and confirmed using a luciferase reporter construct driven by the KSHV Rta ( replication and transcription activator ) promoter , the first promoter activated during KSHV replication .

方法1、用脂质体法，把含有EWS-FLI-1特异结合基因序列的虫荧光素酶报告质粒（pS2）转染到体外培养的尤文肉瘤细胞系（RD-ES、SK-ES）以及对照细胞系（Hela、NIH3T3）。

Methods 1 . The transfection of luciferase reporter plasmid ( pS2 ), which contains the EWS-FLI-1 binding sequence , into cultured Ewing 's sarcoma cell lines ( RD-ES , SK-ES ) and control cell lines ( Hela , NIH3T3 ) was performed by liposome method .

萤火虫荧光素酶报告基因重组逆转录病毒载体的构建及鉴定

Construction of Recombinant Retroviral Vector Containing Firefly Luciferase Reporter Gene

感光受体外周蛋白结合蛋白对虫荧光素酶体外折叠的促进作用

Improvement of luciferase refolding in vitro by peripherin-binding protein

阳离子脂质体介导的虫荧光素酶基因表达

Cationic liposome mediated-transter and expression of firefly luciferase gene

海藻糖对虫荧光素酶活性保护作用研究

Study on the Trehalose Protection on Activity of Luciferase

虫荧光素酶报告基因用于二恶英类化学物质的检测

Detection of Dioxin-type Chemicals by Luciferase Reporter Gene

二恶英反应增强子调控的虫荧光素酶报告基因质粒的构建

Construction of Luciferase Reporter Plasmid Which is Under the Control of Dioxin - responsive Enhancers

目的：构建能用来检测缺氧应答启动子转录调控作用的萤火虫荧光素酶报告载体。

AIM : To construct the firefly luciferase reporter gene vectors regulated by hypoxia response promoters .

选用了两种分别编码绿色荧光蛋白和虫荧光素酶的质粒。

Enhanced green fluorescent protein ( GFP ) plasmid and Luciferase plasmid was selected as the report genes , respectively .