蛋白质研究技术

蛋白质组研究技术主要包括双向电泳、质谱和生物信息学。

Proteomic techniques mainly include two-dimensional electrophoresis , mass spectrometry and bioinformatics .

蛋白质结构研究技术的发展

Research technology progress of protein structure

~（18）O稳定同位素标记定量蛋白质组研究技术的建立与优化

Establishment and Optimization of Stable Isotope ~ ( 18 ) O-labeling Strategy for Quantitative Proteomics Research

各种分析技术的连用和分析过程的自动化将是蛋白质组研究技术的发展方向。

Combined use of various analysis techniques and automation in instrumentation will be the recent trend in this field .

温敏致死（孵化不可能）的分子机制仍不很清楚，本研究采用蛋白质组研究技术，通过不同催青条件下具温敏性状品种的胚胎蛋白质分析，探索温敏不孵化的分子机理。

This research was carried out to explore the molecular mechanism of not-hatching embryo by analyzing the protein of embryo with temperature sensitivity under different incubating condition with proteome technology .

差异蛋白质组学研究技术新进展

Advances in Differential Proteomics Techniques

基于方证相应，运用蛋白质组学研究技术，在蛋白质表达水平上探寻肾阴虚证的本质是可行的。

According to the correspondence of prescription and syndrome , it is feasible that the proteomics research on the essence of kidney-yin deficiency symptoms .

蛋白质组学研究技术作为生命科学里的前沿技术，具有敏感、准确、高通量、快速的优点，为内异症的研究提供了强有力的工具。

Proteomic technologies , as the frontier of life science , provide sensitive , fast and high throughput tools for the research of endometriosis .

本文综述了蛋白质组学研究技术的进展，包括样品制备、蛋白质分离技术、染色技术、蛋白质鉴定技术及蛋白质生物信息学。

In this paper , development of some techniques to proteomics were reviewed , including sample preparation , protein separation , methods of protein solubilization , identification and bioinformatics for proteomics .

为揭示革兰氏阳性中度嗜盐菌的耐盐机制，利用差异蛋白质组学研究技术，得到了高分辨率的双向电泳图谱。

To investigate the salt tolerant mechanisms of the Gram-positive moderately halophilic bacterium D-8 ~ T , the differential proteome techniques was employed and the high resolution 2-D PAGE maps were obtained .

本文介绍了病毒感染蛋白质组学研究技术、病毒诱导宿主细胞蛋白质组改变和病毒感染宿主血清差异蛋白质组等方面的研究进展。

In this paper , techniques of viral infection proteomics , the progress of changes of host cell proteome induced by virus , and serum differential proteome of host after viral infection were introduced .

目的：建立白血病细胞HL60蛋白质组学研究的技术体系。

Objective : To establish the techniques in the proteome research of leukemia cells .

dsDNA芯片（double-strandedDNAmicroarray）是用于DNA结合蛋白质表达研究的新技术，应用一种新的dsDNA芯片制作方法，研究了dsDNA探针设计，开发了相应的计算机模拟系统&DBP软件。

DsDNA ( double-stranded DNA ) microarray , as a novel high-throughout technique , get a start in the field of DNA-binding protein research . It studied a dsDNA probe designing method for a new fabrication technology of the dsDNA microarray .

同位素标记相对和绝对定量（iTRAQ）技术是近年来新开发的一种蛋白质组学定量研究技术，具有较好的定量效果、较高的重复性，并可对多达8种不同样本同时进行定量分析。

Isobaric tags for relative and absolute quantification ( iTRAQ ) is a new quantitative proteomics technology , which has proved to be efficient , reproducible , and able to simultaneously perform quantitative protein analysis of eight samples .

生物质谱&蛋白质组研究的关键技术

Bio-Mass Spectrometry & the Key Technique for the Proteome Research

翻译后修饰蛋白质组学研究的技术策略

Technique Strategies of Modification-specific Proteomics Study

多肽固相合成技术是现代蛋白质化学研究的关键技术，也是目前多肽合成的常规技术。

Solid-phase peptide synthesis ( SPPS ) method is the key technique in modern protein chemistry .

蛋白质组学研究的技术体系及进展

Progress on Proteome Research

生物质谱具有这种特点，因此成为了蛋白质组研究的支撑技术之一。

Biological mass spectrometry ( MS ) has these characters and thus become a supporting technique of proteomic researches .

本研究应用显微镜观察、分子生物学和蛋白质组学的研究技术，对内生根瘤菌与植物相互作用，并促进生长的机制进行了探索。

In this study , we applied a multidisciplinary approach including microscopy ( LCSM ), molecular techniques and proteomics to explore the mechanism of plant growth promotion from their interaction .

然而，由于蛋白质的可变性和多样性等特点，蛋白质组研究在分析技术和分析仪器上还有很多瓶颈问题有待解决。

However , because of the diversity and variability of protein , proteomics studies are far more complex and much more difficult than genomics studies , especially bottlenecks will arise in analytical techniques and instruments .