方法构建含cagA基因片段的重组质粒克隆株，以重组质粒DNA为模板PCR扩增cagA基因片段用长臂光敏生物素标记产物DNA，对病人进行基因探针检测及PCR、尿酶试纸检测。
Methods We cloned the cagA gene fragment in pGEM T vector , and amplified cagA fragment with the template recombinant plasmid by PCR .
酶促试纸条测定唾液中乙醇含量的研究
A Study on Test Paper of Alcohol in Saliva
酶试纸