免疫共沉淀

同时，通过免疫共沉淀和配体竞争实验，我们发现GABAB受体可以与IGF-1受体相互作用并且两者之间的转激活效应是配体非依赖的。

Meanwhile , using co-immunoprecipitation and ligand competition assay , we showed that there was an interaction between GABA_B receptor and IGF-1R while the transactivation of IGF-1R by GABA_B receptor was ligand-independent .

以上免疫共沉淀实验、ChIP检测和基因研究结果表明，hnRNPA2/B1可以选择性结合反转录转座子并且募集具有沉默转座子功能的HP1。

Our results from co-immunoprecipitation experiments , ChIP assays and genetic studies suggest that hnRNP A2 / B1 could bind to selective retrotransposons and recruit HP1 for transposon silencing .

免疫共沉淀实验进一步证实PAI2通过其CD螺旋间区与IRF3在细胞内也存在特异性的相互作用。

Co immunoprecipitation experiments confirmed the interaction between PAI 2 and IRF 3 in vivo .

免疫共沉淀筛选细胞内与A型流感病毒M2蛋白相互作用的蛋白

Screening cellular proteins interacted with M2 protein of influenza A virus by Coimmunoprecipitation

琼脂糖ProteinA反向免疫共沉淀技术纯化高活性BTV方法的建立

Developing a Method of Purifying High Active Bluetongue Virus with Agarose Protein A in Reverse Co-immunoprecipitation

所涉及到的研究方法主要有：细胞核抽提，免疫共沉淀，免疫荧光，Western印记法，构建缺失突变体以及质谱法。

The main assays performed in the study are listed as below : nuclear extract preparation , immunoprecipitation assay , immuno-fluorescence assay , western blotting , deletion construction and mass spectrometry .

利用体外PullDown实验和体内的免疫共沉淀实验，我们发现DJ-1能够在细胞核内与p53的DNA结合区域及C末端相互结合。

Using pull-down and immunoprecipitation experiments , we show that DJ-1 is able to bind to p53 through its DNA-binding domain and C-terminus .

酵母maitrig实验、体外GSTPullDown实验和体内免疫共沉淀实验验证了这些相互作用的特异性。

Yeast mating assay 、 GST Pull-down assay in vitro and co-immunoprecipitation assay in vivo confirmed the specificity of these interactions .

方法采用瞬时转染报告基因测定法，测定细胞裂解液荧光素酶活性（p53的转录活性）或Western-blot、免疫共沉淀测定细胞内p53蛋白水平。

Method Transfection , reporter assay , western blot and autoradiography were performed to determine p53 transcriptional activity and its protein level .

免疫共沉淀实验表明，Sp1和Sp3之间存在着直接的相互作用。

Coimmunoprecipitation suggested that Spl and Sp3 have physical interaction .

免疫共沉淀的实验结果表明c-Abl激酶与αvβ3整合素存在于同一个蛋白复合体中。

Immunoprecipitation experiments showed that the c-Abl kinase and α v β 3 integrin present in one protein complex .

方法采用免疫共沉淀、银染色及Western印迹检测65例人脑肿瘤组织、8例正常人脑组织及2株人脑胶质瘤细胞系中Tag的表达。

Methods Tag was determined by immunoprecipitation , silver staining and Western blot in 65 cases of human brain tumors , 2 human glioma cell lines and 8 cases of normal brain tissues .

酵母双杂交、免疫共沉淀研究禽传染性支气管炎病毒S1、M蛋白相互作用

Research Protein-protein Interaction between S1 Glycoprotein and Membrane Protein of Avian Infectious Bronchitis Virus by Yeast Two-Hybrid System and Co-immunoprecipitation

免疫共沉淀法检测核蛋白中BAG-1与糖皮质激素受体（GR）的相互结合；

The interactions between BAG-1 and glucocorticoid receptor ( GR ) were detected with immune co-precipitation .

应用网织红细胞裂解体外翻译及免疫共沉淀试验，进一步验证CD81分子与HBeAg的结合。

The binding of HBeAg to CD81 was also detected in vitro translation and coimmunoprecipitation test .

琼脂糖蛋白质A反向免疫共沉淀法纯化蓝舌病毒HbC3琼脂糖ProteinA反向免疫共沉淀技术纯化高活性BTV方法的建立

Purifying BTV-HbC_3 by Agarose Protein a Reverse Co-Immunoprecipitation Developing a Method of Purifying High Active Bluetongue Virus with Agarose Protein A in Reverse Co-immunoprecipitation

同时采用荧光素酶双报告基因方法检测了Wnt通路活性，用免疫共沉淀筛选作用于Wnt通路的关键蛋白。

The Wnt pathway activity was detected by luciferase dual reporter gene assay , and the target protein in Wnt pathway was revealed by immunoprecipitation .

免疫共沉淀探讨septin蛋白家族间的相互作用

Co-immunoprecipitation for interactions of three human septin proteins

免疫共沉淀（CoIP）和哺乳动物双杂交实验表明，Sp1和p300通过Sp1的N末端和p300的Q区的相互作用而存在于同一复合物中。

Co-immunoprecipitation ( CoIP ) and mammalian two-hybrid assays revealed that p300 and Sp1 formed a complex through interaction between the Q domain of p300 and the N-terminal domain of Sp1 .

采用外源和内源免疫共沉淀实验我们验证了HPD与NEMO的相互作用，确定HPD通过N端与NEMO的N端发生相互作用，并证明HPD可能与IKK组成蛋白质复合体。

Using in vitro and in vivo coimmunoprecipitation assay , we confirmed the interaction between HPD and NEMO through their N terminals , and HPD may form a protein complex with IKK complex .

所制备的抗体也为相关功能研究，如免疫共沉淀、ChIP-on-chip、Pull-down以及抗逆反应等积累了资源。

The antibodies will also provide resources for functional studies , such as co-immunoprecipitation , ChIP-on-chip , Pull-down and stress response etc.

利用体外转录翻译系统和免疫共沉淀验证了这两种蛋白在体外的相互作用，结果表明KED样蛋白能够在体外与ACaMZ发生相互作用，并且这种作用是c扩+依赖型的。

We tested the interaction of these two proteins using in vitro transcription and translation system and coimmunoprecipitation . It was shown that the two proteins could interact in vitro and the interaction was Ca ~ ( 2 + ) dependent .

方法采用酵母双杂交方法，以人Per1的PAS结构域为诱饵，扫描人下丘脑cDNA文库，并通过体外转录、免疫共沉淀验证蛋白间相互作用；

Method Using hPer1_ ( PAS ) domain as bait , human brain cDNA library was scanned by yeast two-hybrid . The interaction was confirmed by transcription / translation in vitro and co-immunoprecipitation tests .

进一步用染色质免疫共沉淀（ChIP）共定位CMV启动子和RNA聚合酶Ⅱ（RNAPⅡ），PCR结果显示存在MAR元件时，更多的RNA聚合酶Ⅱ被富集到启动子区。

Further , co-localization with newly CMV promoter and RNA polymerase ⅱ( RNAP ⅱ) was detected by chromatin immunoprecipitation ( ChIP ), The PCR result demonstrates that more RNAP ⅱ was recruited to the CMV promoter to initiate transcription in presence of MAR.

方法：以TRF1抗体应用免疫共沉淀方法，从细胞蛋白抽提物中分离TRF1蛋白复合物，并作蛋白质肽指纹谱鉴定；

Methods : The co immunoprecipitation assay was employed to isolate TRF1 protein complex and the immunoprecipitate was subjected to MALDI TOF mass spectrometry for protein identification .

用细菌双杂交及免疫共沉淀法还发现了两种新基因编码蛋白，可以与BRS-3发生相互作用，但是其具体的生物学功能及意义不清楚。

We found two new proteins interacted with BRS-3 , whose biological function and biological significance are not known yet with the bacterial two-hybrid technique and Co-Immunoprecipitation .

为验证这一假说，我们利用免疫共沉淀检测PTHrP和Bmi-1是否能够相互结合，并分析了同窝2周和4周龄Bmi-1基因敲除纯合子小鼠和野生型小鼠皮肤表型的差异。

To test our hypothesis , the interaction of PTHrP with Bmi-1 was assessed by co-immunoprecipitation and the dermatic phenotypes were compared between the wild-type and Bmi-1 knockout ( KO ) mice at 2 and 4 weeks of age using morphological , cellular and molecular approaches .

方法：应用免疫共沉淀法研究蛋白质的相互作用；

Methods The interaction was demonstrated by immuno - coprecipitation .

C6/36细胞上登革2型病毒受体的免疫共沉淀

Immunological Co-sedimentation of Dengue 2 Virus ' Receptor on the C6 / 36 Cells

这与使用生物抗体的免疫共沉淀方法得到的结果相一致。

These results were in accordance with that obtained by co-immunoprecipitation method using antibody .