成肌细胞

MSTNRNAi双元表达载体对成肌细胞增殖及分化的影响

Effects of MSTN RNAi Binary Expression Vector on Myoblast Hyperplasia and Differentiation

成肌细胞移植阻止mdx鼠运动诱导的肌损害

Myoblast transplantation in mdx mice prevents muscle damage by exercise

VEGF（165）转染成肌细胞的效率、蛋白表达、蛋白功能及对成肌细胞增殖、分化的影响作用；

VEGF_ ( 165 ) and the transfection efficiency and protein expression were tested ;

成肌细胞介导的hFⅨ蛋白在小鼠体内的持续表达

Lasting expression of hF ⅸ mediated by myoblasts in mice

胰升糖素样肽1诱导成肌细胞株C2C（12）分化为分泌胰岛素样物质细胞

Glucagon-like peptide-1-induced myoblast line C _2C_ ( 12 ) cells differentiates into cells secreting insulin-like substance

细胞因子IL-1β/IFN-γ对骨骼肌成肌细胞基因表达的影响

Effects of cytokines IL-1 β / IFN - γ on rat skeletal myoblasts

含hFⅨ微小基因的杂合型逆转录病毒载体在小鼠成肌细胞中高效表达的研究

Exploration of high expressions of retroviral vectors containing hF ⅸ minigene in murine myoblast cells

应力介导的Caspase-3家族依赖性体外成肌细胞凋亡的研究

Caspase-3 Mediated Cyclic Stretch Induced Apoptosis in Myoblast in Vitro

其次，我们研究了Caspase-3在成肌细胞的凋亡过程中的作用。

Second , we investigated the effects of Caspase-3 in myoblasts apoptosis .

新生SD大鼠成肌细胞体外培养的实验研究37例口腔颌面部原代鳞状细胞癌的MTT实验

Primary culture of myoblasts of neonatal rat Research of MTT assay of primary culture of oral maxillofacial squamous cell carcinoma

转染VEGF基因的骨骼肌成肌细胞移植结合左心室成形术治疗缺血性心脏病晚期充血性心力衰竭的实验研究

An Experimental Study of Transplantation of Skeletal Myoblasts Transfected by VEGF Gene Combined with Left Ventricular Repair Surgery on Ischemic Congestive Heart Failure

EGFP表达盒通过脂质体法分别转染心肌与骨骼肌成肌细胞。

MHC-EGFP expression cassette was transfected into cardiomyocytes and myoblasts , respectively .

成肌细胞在体外培养不同时间的增殖和分化过程中及加硒均可可引起各基因mRNA表达水平的改变。

Chick embryo myoblasts were cultured for different time in vitro and the addition of Se in the culture system could change all gene mRNA expression detected .

急性骨骼肌钝挫伤修复过程中，转基因成肌细胞注射组VIMENTINmRNA的表达水平始终在3组中表达最低。

During the repairing process , the mRNA expression level of vimentin of transgenic group was the lowest among three groups .

成肌细胞移植治疗DMD模型-mdx鼠骨骼肌Dystrophin表达的实验研究

Dystrophin expression in muscle tissues of DMD model-mdx mouse after myoblast transfer therapy

将巨噬细胞培养上清加入低浓度血清培养的成肌细胞中，作用72h后收集细胞，通过Wright染色、LDH细胞化学和免疫细胞化学方法观察成肌细胞的生长情况。

Cells were harvested at 72 hours later , their growth was observed with Wright staining , LDH cytochemistry and immunocytochemistry .

计算成肌细胞肌管形成率（RMF）评价其分化能力；

The differentiation was tested by the rate of myotube formation ( RMF );

以机械法结合IA型胶原酶与胰蛋白酶的二步酶消化法的改良成年犬骨骼肌成肌细胞培养方法进行细胞原代培养及扩增。

Autologous skeletal myoblasts were procured by using a modified culture method ( two-step digestion method consisting of collagenase IA and trypsin combined with mechanical mincing ) .

小鼠Wnt-1基因真核表达载体构建及其在大鼠成肌细胞中的表达

Construction of mouse Wnt-1 gene eukaryotic expression vector and its expression in skeletal myoblasts of rats

右侧胫前肌注射以F12培养基为载体的成肌细胞为右侧成肌细胞组；

Rats received injection of myoblasts in the right-side tibialis anterior muscle were taken as the right-side myoblast group .

观察原代人胚成肌细胞（Myoblast，Mb）体外培养的分化特点，并对其分化机制进行初步研究。

This study was intended to observe the biological properties of primary human embryonic skeletal myoblasts cultured in vitro and to inquire about the possible differentiation mechanism .

结果：免疫荧光检测VEGF阳性的成肌细胞占85%～90%，ELISA法检测培养液中VEGF浓度平均为214ng/（106cell·24h）。

Results : Immunofluorescence detection showed that 85 % - 90 % myoblasts were VEGF-positive ; ELISA showed that the mean VEGF level was 214 ng / ( 10 ~ 6 cell · 24 h ) in the culture medium .

【结果】采用Ficoll纯化以国产血清培养，可获得高产量高纯度的成肌细胞。

Conclusion Myoblasts could be purified by Ficoll and cultured with DMEM culture liquid contained serum made in our country , in high quantity .

目的：以小鼠C2C12成肌细胞为对象开展血友病乙基因治疗研究。

Objective : To develop experimentally gene therapy for hemophilia B by using C2C12 cells and C3H mice as models .

MyoD所做的就是，导致细胞分化成肌细胞。

And what MyoD essentially does is , it causes cells to differentiate into muscle cells .

采用经皮经冠状静脉植入自体骨骼肌成肌细胞治疗心肌梗死后心肌收缩功能受损：POZNAN试验

Percutaneous trans-coronary-venous transplantation of autologous skeletal myoblasts in the treatment of post-infarction myocardial contractility impairment : The POZNAN trial

方法：鼠骨骼肌成肌细胞在IL-1β/IFN-γ混合液中孵育12h，用抑制性削减杂交法克隆差异表达基因。

Method : Rat skeletal myoblasts were incubated for 12 h with a combination of IL-1 β / IFN - γ and the differentially expressed genes were determined by a suppression subtractive hybridisation ( SSH ) method .

目前，相关研究尚处于起步阶段，并主要集中于针对APA微囊改构，且仅有个别文献报道了相应微囊包裹成肌细胞的体外评价实验。

Currently , research is still in its infancy and focused on the APA microcapsules for reconstructed , and only reported in the literature corresponding to individual microencapsulated myoblasts in vitro evaluation of experiments .

目的观察过量运动对肌营养不良症模型鼠（mdx鼠）骨骼肌的损害作用，以及成肌细胞移植对运动诱导损害肌纤维的保护作用。

AIM : To observe skeletal muscle damage of mdx mice after overload exercise , and protection to muscle damage induced by exercise due to myoblast transplantation ( MTT ) .

【结果】成肌细胞培养后经台盼蓝检查成活率达95%以上，在接种后4～5d增殖达高峰，A、B、C3组培养液对成肌细胞的培养效果无差异。

Result The myoblast cell viability is up to 95 % by Trypan blue , the cell proliferation arrived climax during 4 ~ 5 day after planted . There is no difference between A , B , and C culture liquid .