顶体反应

蛋白激酶C对精子活力和顶体反应的影响

Influence of Protein Kinase C on Motility and Acrosome Reaction of Sperm

结果表明：1.γ－氨基丁酸可明显地诱发人精子顶体反应（P＜0.05）。

The results showed : 1.GABA can induce human sperm acrosome reaction ( P0.05 );

蛋白C抑制剂对人精子顶体反应和顶体素活性的影响

The Effect of Protein C Inhibitor on Human Sperm Acrosome Reaction and Acrosin Activity

从顶体反应率来看，鲜精的获能效果明显优于冻精，二者差异极显著（P＜0.01）。

Acrosome reaction rate indicated fresh sperm was significantly higher than freeze-thawing sperm ( P < 0.01 ) .

PLC在调控信号转导通路中的特性使其在顶体反应、卵子激活中发挥了巨大的作用。

PLC plays important roles not only in sperm acrosome reaction but also in egg activation .

目的探讨白色念珠菌性阴道炎对人精子顶体反应（AR）的影响机制。

Objective : To explore the mechanism of C.albicans vaginitis on acrosome reaction ( AR ) in human sperm .

目的研究人精子的甘露糖受体（MR）表达与顶体反应的关系，以探讨体外获能培养精子的MR表达是否为人精子获能的标志。

Objective To study the correlation between expression of mannose-ligand receptor ( MR ) on capacitated human sperm in vitro and acrosome reaction induced by zona pellucida ( ZP ) .

目的：探讨一氧化氮（NO）对人精子获能及顶体反应（AR）的影响。

Objective : To explore the effect of nitric oxide ( NO ) on human sperm capacitation and acrosome reaction ( AR ) .

PAF对长白猪精子活率及顶体反应率的影响

The influence of PAF on sperm living rate and AR of a long white pig

在精子顶体反应前后，Fβ在精子表面均特异性定位于精子头部赤道区域和顶体内膜区域。

The fertilin beta subunit was immunized localized to both the equatorial region and inner acrosomal membrane region of the sperm membrane during before and after acrosomal reaction .

结论：总体上，Cd诱导公牛精子膜损伤、活动率降低、DNA断裂、以及顶体反应率降低而导致精子功能损伤。

Conlusion : Collectively , Cd induced membrane impairments , lowered motility , DNA breaks and a decreased rate in the acrosome reaction of bull spermatozoa , leading to sperm dysfunction .

延长获能孵育时间对GABA诱发的顶体反应有极显著影响（P<0.01）。

But extending the incubating time of capacitation had significant effect on the AR induced b > GABA ( P < 0.01 ) .

人精子表面ConA受体在顶体反应中的改变

The Change of Receptor for ConA on the Surface of Human Sperm During the Period of Acrosome Reaction

蓝狐精子体外获能及顶体反应实验中，DMSO的浓度应控制在0.5%以下。

The concentration of DMSO should be controlled below 0.5 % in Blue Fox sperm capacitation and acrosome reaction . 4 .

结果EM组腹腔液与精子孵育16h后，EM组精子顶体反应发生率显著低于对照组。

Results Sperm acrosome reaction in EM group was reduced significantly in comparison with the control 16 h after incubation with peritoneal fluid .

0.1%，0.2%，0.5%浓度的DMSO对已进行获能处理的蓝狐精子顶体反应有促进作用。

The acrosome reaction rate of capacitated Blue Fox sperm was promoted by DMSO ( 0.1 % , 0.2 % and 0.5 % ) .

结果体外获能培养人精子的MR表达率与mZPS诱导的顶体反应率无相关性。

Results There is no correlation between expression of MR on capacitated human sperm in vitro and acrosome reaction induced by mZPS .

本文的目的是为了探讨γ-氨基丁酸（GABA）是否可激发人精子顶体反应（AR）。受精能力及其可能的作用方式。

The present study was undertaken to investigate whether GABA induced the acrosome reaction ( AR ) and fertilizing ability , as well as its POssible mode of action in human spermatozoa .

雷公藤单体（TW（19））对豚鼠精子获能、顶体反应和精卵融合的抑制作用

The Inhibition Effects of TW 19 for the Sperm Forward Motility , Capacitation , Acrosome Reaction and Sperm Penetration Ability of Guinea Pig

结论人精子冷藏在4℃EF溶液中1周，不发生获能和顶体反应，重激活后具有与新鲜精子一样的受精能力。

Conclusions Preservation in cold EF solution does not induce human sperm to undergo capacitation and acrosome reaction , and the sperm so preserved for one week possesses comparable fertilizing capaci-ty as fresh sperm does .

牛精子膜SA-30在获能与顶体反应后的定位变化

Distributive changes of SA 30 on bovine sperm membrane after capacitation and acrosomal reaction

目的探讨小鼠睾丸巨细胞病毒（MCMV）感染对附睾尾部精子顶体反应与膜功能的影响。

Objective To explore the effects of testis murine cytomegalovirus ( MCMV ) infection on mature sperm acrosome reaction and spermatic function of membrane in mice .

获能后SA－30的定位及标记强度无明显变化；诱导顶体反应后，顶体区的标记减为弱阳性。

After capacitation , there were no obvious changes on the binding intensity and the distribution of SA 30.By induction of acrosomal reaction , the binding intensity on the acrosomal region decreased and the binding sites may be considered on the inner acrosomal membrane .

目的研究复温温度和白细胞介素1（IL1）对冷冻精子复温后存活率和顶体反应率的影响。

Objective To investigate the effect of thawing temperature and interleukin 1 ( IL 1 ) on human sperm by measuring viability and acrosome reaction rate .

方法先用钙离子载体A23187诱导精子发生顶体反应，然后用水浴超声波和低速离心法分离得到纯牛精子头。

Methods Acrosome reacted bovine spermatozoa heads ( A 23187 induced ) were separated from their tails with ultrasonic treatment and centrifugation .

抗P3抗体可显著影响精子的活动力、顶体反应和穿卵率，可干扰精子受精功能，因此P3肽对人精子受精功能有影响，具有一定的开发前景。

The anti-P3 antibody serum could reduce the motility , acrosome reaction and penetrate capacity of sperm and disturb sperm 's fertilization ability . So , the potential practicability of synthetic P3 peptide needs to be further determined .

哺乳动物卵透明带糖蛋白ZP3（zonapellucida3）是介导精卵初级结合、诱发精子发生顶体反应的关键分子。目前已在精子中发现多种：ZP3结合蛋白。

Mammal zona pellucida protein 3 ( ZP3 ) has been characterized to be responsible for the primary interactions between sperm and egg and inducement of acrosome reaction .

猕猴精子获能和顶体反应过程中Ca~（2＋）和Ca~（2＋）－ATPase定位及咖啡因和dbcAMP对顶体反应的影响

Ultrastructural localization of ca ~ ( 2 + ) and ca ~ ( 2 + ) - atpase activity before and during capacitation and the effects of caffeine and dbcAMP on the rhesus monkey sperm acrosome reaction

结论一定剂量IL1可降低精子存活率和顶体反应率，可能是由于IL1可使Na+K+ATP酶活性降低，而对丙二醛（MDA）无影响。

Conclusion IL 1 could decrease viability and acrosome reaction rate , which might be due to the decrease of Na + K + ATPase activity , whereas is not related to the amount of MDA .

虽然适量的ROS与精子正常生理功能密切相关，但是ROS过量时会引起细胞氧化-抗氧化失衡，对精子造成严重的损伤，降低精子活性、抑制顶体反应、严重影响精子的授精能力。

Appropriate amount of ROS is closely related to the sperm normal physiological function . Nevertheless , excess of ROS will cause sperm oxidation-antioxidant imbalance , which may result in sperm damage , including reduced sperm activity , inhibited acrosome reaction and reduced sperm fertilization ability .