黄嘌呤氧化酶

方法：采用黄嘌呤氧化酶法测定血清中SOD的水平。

Method s : SOD content in serum was determined with xanthine oxidase .

LD检测采用酶法，SOD检测采用黄嘌呤氧化酶法。

LD and SOD are detected each by enzymic method , xanthine oxidase method .

结果通过两种方法的比较，认为黄嘌呤氧化酶法是种比较好的SOD测活方法。

Results Xanthinoxidase method is considered to be a better method in the test .

NO采用硝酸还原酶法测定：SOD采用黄嘌呤氧化酶法测定SOD活力。

Adopt nitric acid reductase method to examine NO content and xanthine oxidase method to examine SOD activity .

比色法测定胞浆内SOD活力（黄嘌呤氧化酶法）、GSH和MDA。

Colorimetric analysis was used to determine SOD activity , GSH and MDA level in cytoplasm .

采用50%Ⅲ度犬烧伤延迟补液模型，以黄嘌呤氧化酶法观察了血浆SOD变化，并以硫代巴比妥酸法测定了血浆MDA含量。

The plasma SOD and MDA content were determined in 50 % ⅲ° burn dogs with delayed fluid replacement .

结果：在黄嘌呤氧化酶法反应体系中，SOD灭活后依然能够检测到SOD比活力值。

Results : In xanthine oxidase method , the specific activity of SOD still could be detected although SOD was inactivated .

结果：脐血丙二醛与黄嘌呤氧化酶、脐血气pH、氧分压、二氧化碳分压、碱剩余、Apgar5分钟评分以及胎龄等各项指标有显著的相关性。

Results : Highly significant correlations were observed between malondialdehyde and xanthine oxidase umbilical pH Pco2 Po2 base excess gestational age and Apgar score at 5 min.

方法用反相蒸发法制备SOD脂质体，采用黄嘌呤氧化酶法检测SOD活力，静脉注射给药后，测定大鼠血中SOD含量变化和不同组织中SOD含量变化。

Methods The liposomes were prepared by reverse phase evaporation method . The activity of SOD was assayed by method of xanthine oxidase .

目的建立适用于高通量筛选的黄嘌呤氧化酶（XanthineOxidase，XO）抑制剂筛选模型，并以此模型进行XO抑制剂筛选。

OBJECTIVE To establish a high-throughput assay for xanthine oxidase inhibitor screening .

于培养第21d采用黄嘌呤氧化酶法测定培养液中SOD含量，用TBA法测定MDA含量。

The contents of SOD and MDA in culture fluid were determined on the 21st day .

目的通过测定慢性乙型肝炎患者血清中的黄嘌呤氧化酶和维生素E水平，旨在揭示慢性乙型肝炎患者体内氧化及抗氧化的失衡情况，以指导、协助慢性乙型肝炎的临床治疗。

Objective : This experiment mainly investigate changes of antioxidation ability in patients with chronic hepatitis B , through measuring xanthine oxidase and vitamin E in the serum .

治疗组与对照组治疗后黄嘌呤氧化酶水平较治疗前均明显下降（P0.05）。

The treatment group and control group after treatment , the yellow purine oxidase level is before treatment are decreased obviously ( P 0.05 ) .

方法：采用小平台水环境法（flowerpot）制作大鼠睡眠剥夺模型，以黄嘌呤氧化酶法测定睡眠剥夺后大鼠额叶、海马、脑干和下丘脑SOD含量变化，并观察黄芪干预对SOD活性的影响。

Methods : SOD activities in frontal lobe , hippocampus , midbrain and hypothalamus of SD rats after sleep deprivation by flower pot were assayed .

鲁米诺购自Sigma公司。抗氧化活性试剂用黄嘌呤氧化酶（Sigma公司）。

Methyl thiazolyl tetrazolium ( MTT ), luminal and anti-oxidative activity reagent xanthine oxidase were all from Sigma Company .

方法：选取原发性高血压患者30例，无高血压健康人28例，分别用硝酸还原酶法、硫代巴比妥酸法及黄嘌呤氧化酶法检测NO、SOD、MDA含量。

Methods : The serum content of NO , MDA and SOD were measured respectively by nitrate reductase , thiobarbituric acid and xanthine oxidase methods .

采用黄嘌呤氧化酶法测定红细胞和肝匀浆SOD活力，以硫代巴比妥酸法测定血清和肝匀浆MDA含量。

SOD activities in RBC and liver were measured by xanthine oxidase method . Serum and hepatic MDA contents were assayed according to thiobarbituric acid method .

采用黄嘌呤氧化酶法和硫代巴比妥（TBA）法检测颗粒细胞的SOD活性和MDA含量。

The SOD activity and the MDA level in granulosa cells were measured by the methods of zanthine hydrocarbonylation and thio-malonylurea . 4 .

方法：光镜下观察组织形态学变化；NO采用硝酸还原酶法测定：SOD采用黄嘌呤氧化酶法测定SOD活力。

Methods : To observe changes of pathology by microscope ; adopt nitric acid reductase method to examine NO content and xanthine oxidase method to examine SOD activity .

NADH使得次黄嘌呤黄嘌呤氧化酶体系和Fe++H2O2体系的ESR信号明显减弱甚至消失；

ESR spectrum of hypoxanthine-xanthine oxidase and Fe + + & H 2O 2 system were significantly reduced or disappear by NADH .

次黄嘌呤黄嘌呤氧化酶（HXXO）是经典的产自由基体系。

Hypoxanthine - xanthine oxidase ( HX-XO ) is a typical system for generating free radicals .

目的考察黄嘌呤氧化酶（XanthineOxidase）在自发性高血压大鼠（SHR）血管舒缩及内皮功能障碍中的作用。

Objective To investigate the role of xanthine oxidase in blood vessel contractility , endothelial dysfunction in spontaneously hypertensive rats ( SHR ) .

7天后测定血清内谷胱甘肽、超氧化物歧化酶（SOD）及血清黄嘌呤氧化酶（XOD）浓度。

Glutathione concentration and levels of xanthine oxidase ( XOD ) and superoxide dismutase ( SOD ) in the serum were determined .

方法：用改良硫代巴比妥法、黄嘌呤氧化酶法和谷胱甘肽氧化酶法对44例急性颅内出血患者血浆LPO，SOD和GSH－PX含量测定，并以26例正常对照。

Methods : 44 cases patients with acute cerebral hemorrhagic disease and 26 controls were determined by Thiobarbituric acid reaction , Xanthine oxidase test and Glutathione oxidase test .

用Massey法从胰酶处理牛乳中提纯黄嘌呤氧化酶，但对其程续进行某些改进。

Massey 's method was modified for the purification of xanthine oxidase from pancreatin-treated milk .

老化血红素对黄嘌呤氧化酶的抑制作用不同，它不影响DCPIP为受体时的活力而却抑制细胞色素c的还原。

In this case , the reduction of cytochrome c and ferricyanide but not the reduction of DCPIP was inhibited by aged haematin .

使用聚苯胺黄嘌呤氧化酶电极能可靠地测定较低的底物浓度，如2×10~（-6）mol·dm~（-3）黄瞟呤。

At a very low concentration of the substrate , such as 2 × 10-6mol · dm-3 , xanthine can be determined reliably by using a polyaniline oxidase electrode .

次黄嘌呤和黄嘌呤氧化酶反应产生的含氧自由基可以引起IAR20肝细胞SCE增高，而GTE可明显抑制这种自由基对细胞SCE的诱导作用。

GTE decreased the frequency of SCE induced by oxygen radical in IAR 20 liver cells treated with hypo-zanthine and xanthine oxydase .

方法：采集30例GDM患者和45例正常孕妇血清，以硫代巴比妥酸（TBA）比色法、黄嘌呤氧化酶法、硝酸还原酶法分别测定MDA，SOD和NO含量。

Methods : Blood samples of 30 GDM and 45 unrelated normal controls were used to measure MDA , SOD and NO levels with methods of TBA , xanthine oxidase and nitric reductase .

用黄嘌呤氧化酶法测定皮肤SOD活力，用硫代巴比妥酸（TBA）法测定皮肤MDA的量，用碱水解法测定皮肤HOP含量，用HE染色法观察皮肤组织形态学改变。

The measurement of SOD vigour , MDA quantity , contents of HyP and the changes of the histomorphology of the skin were by xanthinoxidase , TBA , alkaline hydrolysis and HE staining methods individually .