基因工程

利用基因工程提高植物维生素E营养品质的策略

Improving the vitamin E nutrition of plant by gene engineering

固定CO2基因工程菌的构建

Construction of the gene engineering strains for co_2

我们可以使用基因工程技术把相关基因分离出来。

We can use genetic engineering techniques to isolate the gene that is responsible

C4光合作用的基因工程研究进展

Advances in Genetic Engineering of C_4 Photosynthesis Pathway

基因工程人γ干扰素C端的结构分析及功能作用

Analysis on Function and Structure of C Terminus of the Recombinant Human γ - Interferon

目的利用原核基因工程克隆表达柯萨奇-腺病毒受体（CAR）胞外段，并制备多克隆抗体。

Objective To construct expression plasmid encoding the extracellular domain of human CAR gene by prokaryotic genetic engineering .

随着重组DNA技术的不断完善，基因工程药物的研制和利用日益增多。

As recombinant DNA technology is consummating increasingly , genetic engineering drug is frequently researched and utilized .

基因工程需要使用重组DNA技术，但并不包括传统的种植物育种和诱变育种。

It involves the use of recombinant DNA techniques , but does not include traditional animal and plant breeding or mutagenesis .

用基因工程手段生产HBsAg蛋白

Producing HBsAg protein by the way of genetic engineering

基因工程最简单的形式是从一个有机体内提取出一个基因，将它植入另一个有机物的DNA中。

In its simplest form , genetic engineering involves snipping a gene out of one organism and pasting it into the DNA of another .

目的通过亲和层析的方法纯化基因工程人抗体Fab（Fragment，antigenbinding）片段。

Objective Antigen binding fragments ( Fab ) were purified by affinity chromatography .

MTT法测定三种基因工程细胞生长因子生物学活性方法研究

The Determination for Biological Activities of Three Recombined Human Cell Growth Factors by MTT Method

因此，利用基因工程方法生产hFⅧ无疑具有巨大的商业价值。

Therefore , using genetic engineering method to produce hF ⅷ undoubtedly have great commercial value .

基因工程IFNα（2a）和IFNα（2b）对肿瘤细胞生长的抑制作用

Inhibition Effect of Recombination Interferon α 2a and α 2b on Tumor Cells Growth

丙型肝炎病毒NS3蛋白人源基因工程单链抗体的表达

Expression of human single-chain variable fragment antibody against non-structural protein 3 of hepatitis C virus antigen in E.coli

在获得重组胰高血糖素基因工程菌基础上，利用定点突变技术改造其第21位氨基酸天冬氨酸为丙氨酸，并经DNA测序证明胰高血糖素基因发生了点突变。

Using recombinant glucagon expression system , the 21st aspartic acid was changed to be alanine by means of site directed mutagenesis . Glucagon gene mutation was testified by DNA sequencing .

利用基因工程技术从HeLa细胞中克隆了人端粒酶RNA组分（hTR）基因。

Human telomerase RNA component ( hTR ) gene was cloned from HeLa cells using gene engineering technology .

基因工程乙肝疫苗的研制&Ⅰ.MT-5细胞的HBsAg表达特性初探

Development of Genetic Engineering Hepatitis B Vaccine ⅰ . A Preliminary Study on the Expression Characteristics of HBsAg in MT-5 cells

DNA疫苗作为第三代疫苗，其具备传统疫苗和基因工程苗不可比拟的优点，能够诱导全方位的免疫反应且使用安全方便，开创了疫苗学的新纪元。

As the third generation of vaccine , DNA vaccine has advantages of traditional vaccine and genetic engineer vaccine , which can induce complete immune response and can be used safely and conveniently .

dsRNA介导的烟草抗病毒基因工程研究

Genetic Engineering for Virus Resistance in Tobacco Mediated by dsRNA

用生长抑素基因工程苗（SS）对自然放牧状态下的牦牛、绵羊作免疫增重试验，观察增重效果。

Using SS vaccine immunized yaks and sheep which living in natural conditions , It was to observe its immune and growth effect .

对硝基苯酚降解菌P3的分离、降解特性及基因工程菌的构建

Isolation and Characterization of A p-Nitrophenol Degradation Pseudomonas sp. Strain P3 and Construction of a Genetically Engineered Bacterium

以基因工程表达NP进行4轮吸附-洗脱-扩增的筛选，酶免疫实验检测抗体活性。

Phages from the library were screened by 4 rounds of absorption-elution-propagation with gene-engineering expressed NP and individual clones were assayed by enzyme immunoassay .

可转化人工染色体（TAC）克隆载体及其在果树基因工程研究中的应用前景

Transformation - competent Artificial Chromosome ( TAC ) Cloning Vector and its Application Prospects in Fruit Genetic Engineering

基因工程抗体主要包括单链抗体（ScFv）、Fab抗体、F（ab`）2抗体等小分子抗体、嵌合抗体和改形抗体。

Gene engineering antibodies comprise small molecular antibodies of ScFv antibodies , Fab antibodies , F ( ab ′) 2 antibodies and so on .

结论：获得了具有活性的抗角蛋白的Fab抗体，为其应用研究打下了基础，也表明包涵体表达基因工程抗体技术是可行的。

CONCLUSION : The successfully prepared anti-keratin Fab with binding activity to human keratin laid a solid foundation for its further application .

结论经基因工程方法制备的抗人宫颈癌ScFv具有与亲本单克隆抗体相似的抗体活性和特异性。前列腺癌患者噬菌体抗体库的构建

Conclusions The soluble and phage displayed scFv antibody expressed in E. Construction of Phage - displayed Antibody Library from Patients with Prostatic Carcinoma

心脏基因工程研究对一级康复的干预作用：构建柯萨奇B2病毒毒粒蛋白候选基因疫苗诱导体液免疫的评估

Role of cardiac gene engineering in the primary rehabilitation induction of humoral immunity by constructing virion protein 1 candidate gene vaccine against coxsackievirus B2

同传统的基因工程多肽类药物相比，小分子药物具有易被吸收，没有免疫原性和高亲和力等优势，本实验利用SELEX技术寻获能与TNF结合的寡核苷酸适配子。

Compared with traditional recombinant protein drugs , small molecular drugs have any advantages , such as easy absorbance , no immunity and high affinity etc.

目的：研究一株基因工程人源性抗角蛋白Fab抗体在无血清培养的角质形成细胞中的免疫学活性，观察其对角质形成细胞增殖的影响。

Objective : To investigate the immunological activity and the effect of a genetically engineered human antibody against keratin on the proliferation of serum-free cultured keratinocytes .