基因枪法

基因枪法是小麦遗传转化中最为广泛使用的一种方法，它具有无宿主限制、靶受体类型广泛、可控度高、操作简便快捷等优点。

Gene gun bombardment has been widely used in wheat genetic transformation , for the virtues of no host-limit , wide target receptors , controllable and easy to operate .

果树基因转化方法主要包括农杆菌介导法、电激法、基因枪法、PEG转化法等。

The methods include using Agrobacterium mediated transformation , electroporation , gene gun bombardment and polyethyleneglycol ( PEG ) mediated direct DNA transfer .

用基因枪法将bar基因导入杜氏盐藻及转基因藻株的检测

Transfer of bar gene to Dunaliella salina by microprojectile delivery and detection of transgenic alga

用基因枪法成功地把抗除草剂基因bar导入了4个优良旱稻品种，获得了大量转基因抗性植株。

The gene bar was transferred into 4 elite upland rice cultivars using particle bombardment and lots of plantlets with herbicide tolerance were obtained .

以基因枪法将Bt杀虫蛋白基因导入常规玉米自交系的研究

Studies on Transferring Insecticidal Protein Gene from Bacillus Thuringiensis into Excellent Maize Inbred-line via Microprojectile Bombardment

基因枪法将LEAFY基因导入甘蔗的研究

The Study on Transformation of LEAFY Gene into Sugarcane by Particle Bombardment

利用基因枪法将Bt基因导入早熟玉米优良自交系

Transfer of Bt gene into early maturing maize elite inbred line 501 and C_ ( 111 ) by microprojectile bombardment

本试验利用基因枪法和花粉管通道法两种植物遗传转化技术，将叶片衰老抑制基因P（SAG12）-IPT导入有早衰现象的小麦品系9848，获得转基因植株。

In this study , transgenic wheat plants of 9848 with P_ ( SAG12 ) - IPT gene were obtained by micro-projectile bombardment and pollen tube pathway .

作者通过根癌农杆菌（Agrobacteriumtumefaciens）Ti质粒介导和基因枪法，将含抗除草剂Bar基因的植物表达载体，导入小麦幼穗和幼胚愈伤组织。

The plant expression vector carrying a herbicide resistance BAR gene and a marker GUS gene was transferred into wheat spikelets and immature embryo calli via Agrobacterium tumefaciens Ti plasmid and microprojectile bombardment .

用基因枪法将小麦病程相关蛋白基因TaPR-1导入小麦的研究

Transferring Wheat Pathogenesis-related Proteins 1 ( TaPR-1 ) Gene into Wheat via Micro-projectile Bombardment

用基因枪法将含有抗除草剂bar基因的植物表达载体导入优良品种小麦幼胚愈伤组织，并通过GUS瞬时表达对沉淀剂、轰击距离、金粉用量等轰击参数进行了优化。

The plant expression vector carrying the herbicide resistance bar gene was introduced into wheat immature embryo calli using particle bombardment . Parameters of bombardment were optimized by GUS transient expression , such as precipitants , distance , and amount of gold powder .

采用室内模拟盐、旱胁迫，结合田间实际测定的方法，对基因枪法获得的转BADH基因小麦多个株系的不同世代材料进行了耐盐、耐旱性鉴定。

Assay for salt tolerance and drought resistance of transgenic wheat expressing betaine aldehyde dehydrogenase , obtained through particle gun method , were conducted under simulated salt / drought stress condition .

在云南2种生态条件下，对基因枪法获得的转溶菌酶基因水稻高代品系D2-1-2和受体品种中花9号的农艺性状、稻米蛋白质含量、氨基酸含量和11种矿质元素进行了对照分析。

With trans-lysozyme gene rice line D2-1-2 and its restorer Zhonghua No. 9 as test materials , this paper studied their agronomic characters and grain qualities under two ecological conditions of Yunnan .

转化方法以叶盘法为主，辅助使用了基因枪法。

Leaf-disc method and microprojectile bombardment were adopted for gene transformation .

目前常用的方法是基因枪法和农杆菌介导法。

Particle bombardment and agrobacterium-mediated techniques are commonly used these days .

用基因枪法将抗除草剂基因导入小麦栽培品种的研究

Studies on the Transfer of Herbicide-resistance Gene into Cultivated Wheat by Biolistics

基因枪法向小麦导入几丁质酶基因的研究

Studies on transferring chitinase gene into wheat via microprojectile bombardment

应用基因枪法获得抗大麦黄矮病毒转基因小麦

Transgenic wheat resistant to BYDV were obtained by biolistics

草菇基因枪法遗传转化体系的建立

The Transformation System for Volvariella volvacea by Particle Bombardment

基于壳聚糖纳米颗粒的基因枪法转化洋葱细胞研究

Chitosan Nanoparticle as Gene Vehicle Mediated by Particle Bombardment in Onion Cell Transformation

基因枪法转化小麦及转基因植株的获得

Wheat Transformation by Microprojectile Bombardment and Transgenic Wheat Regeneration

基因枪法转化基因在小麦条锈菌中的瞬时表达

Transient Expression of Transformed Genes by Particle Bombardment

基因枪法转化棉花胚性愈伤组织获得转基因植株

Genetic Transformation of Cotton Embryogenic Callus by Particle Bombardment and Regeneration of Transgenic Plants

基因枪法转化水稻的研究进展

The Studies on Rice Transformation by Particle Bombardment

基因枪法转基因技术的研究综述

Research on particle gun transgenic technique

基因枪法是小麦转基因最常用的方法，其转基因体系已基本建立。

The system of particle bombardment for wheat genetic transformation has been established and was most widely used .

基因枪法转化香蕉薄片外植体的参数优化

Optimization of the Biological and Physical Parameters of Bombardment - Mediated Transformation of Micro - Cross Section Explants of Banana

利用基因枪法，以菜豆几丁质酶基因转化小麦幼胚愈伤组织。

Using embryogenic calli from young embryos as explants , bean chitinase gene was transferred into spring wheat plants by microprojectile bombardment .

至今，基因枪法以其实用性和有效性应用于水稻工程育种已有十几年。

Biolistics , by virtue of its overwhelming practicality and effectiveness , has been applied in rice engineering breeding for a dozen years .

本文就近几年来根农癌杆菌介导法、基因枪法、子房注射法、花粉管通道在玉米基因转化中的应用情况加以综述。

The paper summarized the application of agrobacterium tumefaciens mediated , particle bombardment , ovary injection and pollen tube pathway method in maize transformation .