完全培养液

24h后全量换液，接种3d后，再次全量换液，加入含不同浓度大豆磷脂的完全培养液，继续培养。

The whole medium was changed after 24 h , 3 d after inoculation , the whole medium was changed again , adding different concentrations of soybean phospholipids containing complete medium and continue to develop .

损伤组，培养第7天神经元用微量移液器塑料滴头行机械性划痕损伤后完全培养液培养。

After spinal neurons were cultured for 7 days , mechanical injury was caused by plastic tip in the injury group and then the cells were cultured in complete medium continually ;

以完全培养液为对照组，研究不添加尼罗罗非鱼血清、β-巯基乙醇、bFGF及胰岛素四种添加物时，培养液对ECs增殖的影响。

The whole culture medium was used as the control , the effects of the culture medium without the tilapia perch serum , the bFGF , the 2-ME or the insulin on the proliferation of the ECs were researched .